The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats
2023
Аутори:
Popović, DušankaMirkov, Ivana
Popov Aleksandrov, Aleksandra
Tucović, Dina
Kulaš, Jelena
Malešević, Anastasija
Glamočlija, Jasmina
Soković Bajić, Svetlana
Brdarić, Emilija
Tolinački, Maja
Golić, Nataša
Остала ауторства
Capurso, LucioGuarino, Alfredo
Gasbarrini, Antonio
Morelli, Lorenzo
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2023 EMEC, Roma
Метаподаци
Приказ свих података о документуАпстракт:
Objective:
The little data of the effect of pulmonary fungal infection on lung and
gut microbiota exist, thus aim of our study was to examine the effect of
Aspergillus fumigatus infection on bacterial homestasis in these organs in
Dark Agouti (DA) and Albino Oxford (AO) rats known to develop different
immune responce to the fungus.
Methods:
16S rRNA amplicon sequencing of the V3-V4 hypervariable region.
Enzyme-linked immunosorbent assay for cytokine content (IL-10, IL-6, TNF,
IL-1beta, IL-17 i IFN-gamma) in lung and gut homogenats, and plasma (IL-
6, IL-17, TNF). Myeloperoxidase, catalase, glutathione S-transferase and
content of reduced glutathione in lung homogenates.
Results:
No initial differences in lung bacterial microbiota between healthy DA
and AO rats exists, but A. fumigatus infection resulted in increase in
alpha diversity (without effect on bacterial composition) in both strains.
Changes in relative abundance at genus and species level were expressed
differently (increased in DA, decreased in AO). Dysbiosis in lung coincided
with increase of IFN-gamma, IL-17 and MPO in both strains, and increase
oxidative stress solely in DA. Gut inflammation was noted in both strains,
while bacterial dysbiosis only in DA.
Conclusions:
Pulmonary fungal infection resulted in bacterial dysbiosis in lung (in both
rat strains), and gut (solely in DA). Noted lung and gut inflammation in
these two rat strains, coexisted with different effects on bacterial
homeostasis, which could be due to different genetic background or
bacterial composition prior infection.
Кључне речи:
Aspergillus fumigatus; lung infection; gut microbita; rats; inflammationФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
У:
- Capurso L, Guarino A, Gasbarrini A, Morelli L, editors. 12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023 Sep 16-19; Rome, Italy. Roma: EMEC; 2023. p. 90.