Induction and development of somatic embryos from spinach (Spinacia oleracea) leaf segments
1999
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 1999 Kluwer Academic Publishers
Метаподаци
Приказ свих података о документуАпстракт:
Somatic embryogenesis was induced in embryogenic calli of Spinacia oleracea L., on a Murashige and Skoog (1962) medium, containing 4.6 μM kinetin as the sole growth regulator. Abscisic acid, gibberellic acid, and indole-3-acetic acid were supplemented to kinetin-containing medium and their effects on the initiation of somatic embryos was studied. Abscisic acid at a particular concentration (4 μM) dramatically increased the number of embryos per g fresh weight of callus, while both gibberellic acid and indole-3-acetic acid suppressed the embryo initiation. It is suggested that the promoting effect of abscisic acid on the embryo initiation may be explained as a stress response of the tissue. The relative number of globular embryos vs. the embryos in heart/torpedo and cotyledonary stages was increased at 4 μM abscisic acid and at all gibberellic acid concentrations (0.3–10 μM). In contrast, the ratio of globular to polar embryos was lower than in controls at 1 μM abscisic acid and indole-3-acetic acid (1 and 10 μM). The effects of growth regulators on the ratio of globular to polar embryos indicate that they interfere with the normal distribution of cell division and cell expansion during early embryogenesis.
Кључне речи:
Chenopodiaceae; globular embryos; growth regulators; polar embryos; somatic embryogenesisИзвор:
Plant Cell Tissue and Organ Culture, 1999, 55, 2, 109-114Финансирање / пројекти:
- Ministry of Science and Technology of Serbia, Grant No. O3E21.
DOI: 10.1023/A:1006258426969
ISSN: 0167-6857