Secondary regeneration in somatic embryos of Aesculus carnea Hayne
2006
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Authors:
Zdravković-Korać, SnežanaĆalić, Dušica
Budimir, Snežana
Janošević, Dušica
Vinterhalter, Branka
Radojević, Ljiljana
Contributors
Rakonjac, LjubinkoDocument Type:
Conference object (Published version)
,
© 2006 by the Institute of Forestry, Belgrade, Serbia
Metadata
Show full item recordAbstract:
Somatic embryos of red horse chestnut were subjected to an induction of secondary regeneration. The embryos were divided in 4 classes on the basis of their size (I 1, II 5, III 10 and IV 30 mm), and sub-cultivated on MS media containing 0, 1, 5 or 10 μM kinetin (Kin) or benzyladenine (BA). The pathway of secondary regeneration (somatic embryogenesis or caulogenesis) depended solely on the primary somatic embryo (PSE) stage of development. The PSE of the I and II class produced solely secondary somatic embryos (SSE), the III class PSE formed SSE on media containing Kin, and both SSE and adventitious buds on media containing BA, whereas the IV class PSE developed almost solely adventitious buds. BA promoted bud induction at much higher rate than Kin and slightly higher embryogenic response. The histological study confirmed these findings.
Keywords:
adventitious buds; red horse chestnut; secondary somatic embryosFunding / projects:
- Regulacija morfogenetskih procesa i sekundarnog metabolizma i genetičke transformacije biljaka u kulturi in vitro (RS-MESTD-MPN2006-2010-143026)
In:
- Rakonjac Lj, editor. Proceedings: International scientific conference sustainable use of forest ecosystems: the challenge of the 21st Century; 2006 Nov 8-10; Donji Milanovac, Serbia. Belgrade: Institute of Forestry; 2006. p. 513-8.