Effect of NaCl on chlorophyll and protein content, and antioxidant enzymes activity in two bryophyte species – Atrichum undulatum (Hedw.) P. Beauv. and Marchantia polymorpha L.
2009
Authors:
Bogdanović, MilicaSabovljević, Aneta
Sabovljević, Marko
Dragićević, Milan
Platiša, Jelena
Grubišić, Dragoljub
Document Type:
Conference object (Published version)
,
© 2009 by the Faculty of Biology, University of Belgrade
Metadata
Show full item recordAbstract:
Bryophytes are thought to avoid salty substrates, although in few species reported salt tolerance is greater than in non-halophyte tracheophytes. To explore bryophyte salinity tolerance, two species were grown in vitro on half-strenght MS media with added NaCl (concentrations of 100, 250 and 500 mM). Chlorophyll content was measured spectrophotometrically, and protein content, peroxidase and catalase activities were assayed both spectrophotometrically and by PAGE. Chlorophyll content in M. polymorpha decreases rapidly with rising salt concentrations approaching zero at 250 mM, while in A. undulatum it remains high at 100 mM of salt above which decreases. Protein content remains high at 100 mM of salt in both species and decreases at higher salt concentrations (in M. polymorpha several times compared to control values). Peroxidase activity is much higher in M. polymorpha and decreases slightly at high salt concentrations. Catalase activity in A.undulatum remains high at all salt concentrations, in contrast with M. polymorpha whose activity lowers considerably at 250 mM of salt.
Keywords:
Bryophytes; salt tolerance; NaClFunding / projects:
- Svetlosna i hormonalna kontrola rastenja i razvića biljaka, razmnožavanje in vitro i ex situ zaštita retkih i ugroženih vrsta (RS-MESTD-MPN2006-2010-143031)
In:
- Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia. Belgrade: University of Belgrade, Faculty of Biology; 2009. p. 87.