Uticaj NaCl na morfogenezu i sadržaj hlorofila tri vrste briofita u kulturi in vitro

Abstract

Briofite su poznate kao organizmi koji izbegavaju slane supstrate a u morima ih uopšte nema. S ciljem izučavanja odnosa briofita prema slanoj sredini, gametofiti mahovina Atrichum undulatum, Physcomitrella patens i jetrenjače Marchantia polymorpha su gajeni na MS hranljivoj podlozi sa dodatkom različitih koncentracija NaCl (50, 100, 250, 350, 500mM) tokom 3 nedelje u uslovima dugog dana (fotoperiod 16/8h). Preživljavanje i multiplikacija izdanaka se drastično smanjuju kod sve tri vrste na visokim koncentracijama soli, a produkcija biomase kod M. polymorpha gotovo eksponencijalno opada sa povećanjem koncentracije soli. P. patens dobro preživljava na koncentracijama soli nižim od 350mM. Koncentracija hlorofila a, b i ukupnog hlorofila kod sve tri ispitivane vrste opada sa porastom koncentracije NaCl, a blagi porast količine navedenih pigmenata uočava se na podlozi obogaćenoj sa 100mM NaCl. M. polymorpha vrlo loše podnosi koncentracije soli veće od 250mM u pogledu svih ispitivanih parametara.

Bryophytes are known to avoid salty substrates and do not inhabit the seas. With the purpose of studying the relation between bryophytes and salty environment, gametophytes of the two mosses - Atrichum undulatum, Physcomitrella patens and the liverwort Marchantia polymorpha were grown on MS media enriched with various concentrations of NaCl (50, 100, 250, 350, 500mM) during three weeks at 16/8h photoperiod. Survival and bud formation were drastically reduced in all three species at high salt concentrations and biomass production in M. polymorpha declined almost exponentially as salt concentration increased. P. patens survived concentrations less than 350mM salt quite well. Chlorophyll content (-a, -b and total) in all examined species declined as salt concentration increased, however a modest rise in chlorophyll content in all three species was observed when the concentration of salt in the medium was 100mM. M. polymorpha did not tolerate well concentrations above 250mM according to all the parameters observed.