Hydrolysis of secoiridoid glycosides from Centaurium erythraea Rafn increases their antioxidative potential
2015
Аутори:
Božunović, JelenaŽivković, Suzana
Glamočlija, Jasmina
Božić, Dragana
Aničić, Neda
Šiler, Branislav
Soković, Marina
Mišić, Danijela
Остала ауторства
Uzelac, BrankaТип документа:
Конференцијски прилог (Објављена верзија)
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© 2015 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
Dominant secondary metabolites of common centaury (Centaurium erythraea Rafn) are bitter taste secoiridoid glycosides: swertiamarin, gentiopicrin and sweroside, and their function is proposed to be in plant responses against pathogens and herbivores. It can be assumed that secoiridoid glycosides and hydrolytic enzymes (β-glucosidases) form a dual defense system in common centaury, where β-glucosidase plays an essential role in removing non-reducing terminal glucosyl residues from glycosylated compounds, leading to highly active but unstable aglycones. The present study was designed to evaluate antioxidative and antimicrobial activity of hydrolyzed and non-hydrolyzed methanol extracts of Centaurium erythraea aboveground parts and its main components sweroside, swertiamarin and gentiopicrin. To examine the concentrations of secoiridoid glycosides and their aglycones in methanol extracts, before and after hydrolysis, UHPLC/DAD/+HESI-MS/MS method was developed and evaluated. Hydrolysis was performed enzymatically using commercial β-glucosidase isolated from almond. Results of FRAP, ABTS and DPPH assays showed higher antioxidative activity of hydrolyzed C. erythraea methanol extract and pure compounds than non-hydrolyzed ones. Conversely, hydrolysis of C. erythraea methanol extracts led to lower antifungal activity and had weak or no influence on antibacterial activity. Based on this study it can be presumed that biosynthesis of secoiridoid glycosides, and their degradation mediated by β-glucosidases are regulated by various biotic factors, and are involved in defense system against herbivores and pathogens.
Кључне речи:
secoiridoid glycosides; β-glucosidase; Centaurium erythraeaФинансирање / пројекти:
- Физиолошка, хемијска и молекуларна анализа диверзитета одабраних ретких и угрожених биљних врста у циљу еx ситу заштите и продукције биолошки активних једињења (RS-MESTD-Basic Research (BR or ON)-173024)
У:
- Uzelac B, editor. Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”; 2015. p. 70-1.