Chemical composition and antioxidant activity of three endemic Nepeta species methanol extracts
2015
Authors:
Nestorović Živković, JasminaŽivković, Suzana
Dmitrović, Slavica
Šiler, Branislav
Aničić, Neda
Matekalo, Dragana
Mišić, Danijela
Contributors
Uzelac, BrankaDocument Type:
Conference object (Published version)
,
© 2015 by the Serbian Plant Physiology Society
Metadata
Show full item recordAbstract:
Methanol extracts of in vitro grown shoots of three Nepeta species (Nepeta rtanjensis Diklić & Milojević,
N. sibirica L. and N. nervosa Royle ex Bentham) were studied for their main secondary metabolites using UHPLC/DAD/±HESI-MS/MS analysis. Results showed that the major terpene present in the samples was iridoid monoterpene nepetalactone, wherein trans,cis- isomer was mostly present in methanol extracts of N. rtanjensis, while cis,trans-nepetalactone prevailed in N. sibirica. N. nervosa contained nepetalactone only in traces. Among phenolic acids, rosmarinic acid was predominant in all investigated species, while chlorogenic, neochlorogenic and caffeic acids were present in significantly lower concentrations. The results of ABTS and DPPH assays showed that methanol extracts of N. rtanjensis, N. sibirica and especially N. nervosa possess considerable antioxidant activities, and the FRAP assay revealed high ferric reducing capacity for all the tested samples. Significant antioxidant activity was attributed to phenolic acids, particulary to rosmarinic acid. However, nepetalactone did not contribute notably to the antioxidant potential of the methanol extracts.
Keywords:
Nepeta rtanjensis; Nepeta sibirica; Nepeta nervosa; antioxidant activityFunding / projects:
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
In:
- Uzelac B, editor. Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”; 2015. p. 82-3.