Application of Tempo EPR spin probe for in vivo detection of salt-induced oxidative stress in Centaurium erythraea Rafn
2015
Authors:
Novaković, LazarŽivković, Suzana
Popović-Bijelić, Ana
Mišić, Danijela
Mojović, Miloš
Contributors
Uzelac, BrankaDocument Type:
Conference object (Published version)
,
© 2015 by the Serbian Plant Physiology Society
Metadata
Show full item recordAbstract:
Electron paramagnetic resonance spectroscopy (EPR) was applied for in vivo detection of oxidative stress induced by high ionic strengths in Centaurium erythraea Rafn (Gentianaceae), a herbaceous perennial often found on saline soils. Shoots of C. erythraea were cultured in vitro for 4 weeks on solid 1/2 MS medium, or 1/2 MS medium supplemented with 200 mM NaCl to induce salt stress and thus stimulate the production of reactive oxygen species (ROS), which is a common response of plant cells subjected to various types of biotic or abiotic stresses. The reduction of a stable cell-permeable aminoxyl radical spin probe, Tempo, was measured to assess the oxidative status of the control and salt-treated samples. In both types of samples, the reduction of Tempo showed zero-order kinetics. After one hour, the control reduced only 9% of the initial amount of the spin probe, whereas the sample grown with 200 mM NaCl reduced it by 20%. This may indicate that the plants grown in the presence of 200 mM NaCl produced higher amounts of ROS which are able to reduce the aminoxyl radical. The obtained results indicate that EPR can be used as a method for in vivo evaluation of the redox state of plants under stress conditions, and could also be useful in determining the salt-stress tolerance of plants. Further
studies, including different salt concentrations, and plant species, have to be conducted to verify these findings.
Keywords:
Centaurium erythraea; EPR; Tempo; oxidative stress; salt stressFunding / projects:
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
- Biomarkers in neurodegenerative and malignant processes (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41005)
In:
- Uzelac B, editor. Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”; 2015. p. 178-9.