Volatile compounds of golden fern (Asplenium ceterach L.) detected during the process of rehydration
2015
Аутори:
Živković, SuzanaSkorić, Marijana
Ristić, Mihailo
Perišić, Mirjana
Puač, Nevena
Malović, Gordana
Petrović, Zoran Lj
Остала ауторства
Uzelac, BrankaТип документа:
Конференцијски прилог (Објављена верзија)
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© 2015 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
Volatile organic compounds (VOC) emitted by fronds of desiccation tolerant fern Asplenium ceterach L. during the rehydration process were investigated. Head-space GC-MS analysis was used in order to determine the volatile compounds in dry and rehydrated sample of golden fern. It was found that the VOC profile of golden fern is based mainly on isomeric heptadienals (over 25%) and decadienals (over 20%), other linear aldehydes, alcohols and related compounds. Aerial parts of fresh and dry fronds do not contain monoterpene-, sesquiterpene- and diterpene-type hydrocarbons or corresponding terpenoids. In order to determine the composition of the VOC during the process of rehydration, we have applied proton-transfer reaction mass spectrometry (PTR-MS). PTR-MS is a sensitive technique that allows real-time detection of VOCs emitted from plants. We have used dry plants to establish a base line and then distilled water was added to induce hydration process of the golden fern plant. Masses in the range from m/z 21 to m/z 300 were measured with dwell time of 200 ms and for the time period of 24 hours. The experiment was repeated for five plants. PTR-MS measurements revealed that the amounts of compounds, determined by head-space GC-MS, generally exhibit a significant increase after addition of water. After about 20 hours the values measured by PTR-MS for these masses are reduced to a new base line that corresponds to the rehydrated plant.
Кључне речи:
golden fern; rehydration; PTR-MS; headspace GC-MSФинансирање / пројекти:
- Примене нискотемпературних плазми у биомедицини, заштити човекове околине и нанотехнологијама (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41011)
- Физиолошка, хемијска и молекуларна анализа диверзитета одабраних ретких и угрожених биљних врста у циљу еx ситу заштите и продукције биолошки активних једињења (RS-MESTD-Basic Research (BR or ON)-173024)
У:
- Uzelac B, editor. Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”; 2015. p. 187.