Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme
2023
Аутори:
Aničić, NedaMatekalo, Dragana
Skorić, Marijana
Božunović, Jelena
Nestorović Živković, Jasmina
Gašić, Uroš
Milutinović, Milica
Dmitrović, Slavica
Petrović, Luka
Filipović, Biljana
Banjanac, Tijana
Šiler, Branislav
Anđelković, Boban
Dimitrijević, Milena
Mišić, Danijela
Остала ауторства
Spasojević, IvanТип документа:
Предавање (Објављена верзија)
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© 2023 by the Faculty of Chemistry, University of Belgrade
Метаподаци
Приказ свих података о документуАпстракт:
Nepeta L. holds a prominent position as the largest genus in the Lamiaceae family and serves as the exclusive representative of the iridoid-lacking Nepetoidae subfamily which produces iridoids. The genus showcases a rich chemodiversity, encompassing taxa both with and without iridoids. Our study investigates the genetic basis of iridoid diversity using omics-guided and functional genomics approaches. We identified functional iridoid synthases (ISY) in iridoid-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in iridoid-lacking N. nervosa L. (NnIS). Remarkably, N. nervosa carries a dormant iridoid biosynthetic platform, suggesting a loss of iridoid production during its evolutionary history. Moreover, we explore regulatory mechanisms through comparative iridoid profiling and co-expression analysis of biosynthetic genes and transcription factors (TFs) under various stress conditions (e.g., dehydration, UV − B radiation, pathogens) or elicitors (MeJA). These mechanisms influence plant productivity and the presence/absence of iridoids or their specific groups (iridoid aglycones and iridoid glycosides). Our research offers valuable insights into the molecular mechanisms driving iridoid biosynthesis and the chemical evolution of iridoids within Nepeta. This study enhances our understanding of the intricate relationship between genetics and the environment, providing comprehensive insights into iridoid metabolism, chemical evolution, and ecology.
Финансирање / пројекти:
- NEPETOME - Omics-guided disentangling of the iridoid diversity in the genus Nepeta L. for in planta metabolic engineering towards sustainable exploitation (RS-ScienceFundRS-Ideje-7749433)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- Spasojević I, editor. Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia. Belgrade: Faculty of Chemistry; 2023. p. 39.