Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)
2016
Аутори:
Stojičić, DraganaTošić, Svetlana
Slavkovska, Violeta
Zlatković, Bojan
Budimir, Snežana
Janošević, Dušica
Uzelac, Branka
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2016, Springer-Verlag Berlin Heidelberg
Метаподаци
Приказ свих података о документуАпстракт:
The content and chemical composition of Micromeria
pulegium (Rochel) Benth. essential oils were studied in
native plant material at vegetative stage and in micropropagated
plants, obtained from nodal segments cultured
on solid MS medium supplemented with N6–benzyladenine
(BA) or kinetin at different concentrations, alone or in
combination with indole-3-acetic acid. Shoot proliferation
was achieved in all treatments, but the highest biomass
production was obtained after treatment with 10 μM BA.
Phytochemical analysis identified up to 21 compounds in
the essential oils of wild-growing and in vitro cultivated
plants, both showing very high percentages of total
monoterpenoids dominated by oxygenated monoterpenes
of the menthane type. Pulegone and menthone were the
main essential oil components detected in both wildgrowing
plants (60.07 and 26.85 %, respectively) and
micropropagated plants grown on either plant growth regulator-
free medium (44.57 and 29.14 %, respectively) or
BA-supplemented medium (50.77 and 14.45 %, respectively).
The percentage of total sesquiterpenoids increased
in vitro, particularly owing to sesquiterpene hydrocarbons
that were not found in wild-growing plants. Differences in
both content and the composition of the essential oils
obtained from different samples indicated that in vitro
culture conditions and plant growth regulators significantly
influence the essential oils properties. In addition, the
morphology and structure of M. pulegium glandular trichomes
in relation to the secretory process were characterized
for the first time using SEM and light microscopy,
and their secretion was histochemically analyzed.
Кључне речи:
Axillary bud induction; Capitate trichomes; Histochemistry; Micropropagation; Peltate trichomes; Phytochemical analysisИзвор:
Planta, 2016, 244, 2, 393-404Финансирање / пројекти:
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-MESTD-Basic Research (BR or ON)-173015)
- Испитивање лековитог потенцијала биљака: морфолошка, хемијска и фармаколошка карактеризација (RS-MESTD-Basic Research (BR or ON)-173021)
- Биодиверзитет биљног света Србије и Балканског полуострва - процена, одрживо коришћење и заштита (RS-MESTD-Basic Research (BR or ON)-173030)
DOI: 10.1007/s00425-016-2513-7
ISSN: 0032-0935
PubMed: 27074837