Flower color morphs of Iris pumila differ in the amount of heat shock protein 70 and pigments with antioxidant properties
2017
Preuzimanje 🢃
Autori:
Hočevar, KatarinaNolić, Vladimir
Budečević, Sanja
Manitašević Jovanović, Sanja
Ostala autorstva
Brajušković, GoranĐorđević, Ana
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
,
© 2017 University of Belgrade, Faculty of Biology
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Introduction: Natural populations of the dwarf bearded iris, Iris pumila, are markedly
polymorphic for flower color. Selection pressures exerted by a range of abiotic and
biotic factors could be involved in maintaining the polymorphism. Here we
quantified the amount of the Hsp70 and the two groups of pigments with
antioxidative properties: anthocyanins and carotenoids.These molecules impact
abiotic stress tolerance, ultimately influencing the fitness of individual plants.
Methods: A total of fifty genotypes raised in a common-garden and belonging to a
corresponding color class (yellow, blue, violet, dark blue and dark violet) were
surveyed. One fully developed leaf and a flower from each genotype were
analyzed for the Hsp70 amount and pigments concentration.
Results: The Western blot analysis revealed the presence of one isoform for the Hsp70
in leaf and two isoforms in flower organs. In both vegetative and reproductive tissues
the amount of Hsp70 was found to be the lowest in yellow colored genotypes
compared to other color classes. In violet and blue flowers, the concentration of
Hsp70 decreased gradually from light to dark colored variants. Conversely, the
concentration of anthocyanins was found to be higher in darker than in the lighter
color morphs. An inverse trend was observed for the total carotenoids
concentration.
Conclusion: This study revealed that each I. pumila color genotype produces a
unique amount of Hsp70 and antioxidative pigments in both the vegetative and
reproductive plant parts, in order to protect cellular homeostasis under fluctuating
temperature conditions prevailing in its population.
Ključne reči:
flower color polymorphism; Iris pumila; Hsp70; anthocyanins; carotenoids; flower organsFinansiranje / projekti:
- Evolucija u laboratoriji i adaptacije u prirodi (RS-MESTD-Basic Research (BR or ON)-173007)
U:
- Brajušković G, Đorđević A, editors. Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia. Belgrade: University of Belgrade, Faculty of Biology; 2017. p. 39.