Changes of antioxidative enzymes in Impatiens walleriana L. shoots in response to genetic transformation
Promene antioksidativnih enzima u izdancima Impatiens walleriana L. kao odgovor na genetičku transformaciju
2015
Authors:
Milošević, SnežanaTrajković, Milena
Antonić Reljin, Dragana
Cingel, Aleksandar
Subotić, Angelina
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Impatiens walleriana L. shoots were inoculated with Agrobacterium rhizogenes
A4M70GUS and the effects of genetic transformation on the catalase (CAT), superoxide
dismutase (SOD) and peroxidase (POX) activities in wounded region of stems and
unwounded leaves were evaluated 10, 24, 240 and 720 hours after inoculation. Following
Agrobacterum infection activities of plant antioxidative enzymes changed in a timedependent manner indicating that dynamic processes occurred during plantAgrobacterium interaction, plant cell transformation and formation of hairy roots.
Appearance of hairy roots on wound sites of shoots was observed ten days after
inoculation with A. rhizogenes and the root induction frequency was 100%. Among
selected hairy root lines significant differences in growth rate and biomass production
were observed and an average 3-fold increase in biomass production was observed for the
best growing hairy root line compared with the untransformed roots. PCR analysis
showed presence of uidA, rolB, rolC and rolD genes in all analyzed I. walleriana L. hairy
root lines, while amplification fragment of rolA gene was detected in 83.3% transformed
lines. Efficient transformation protocol for I. walleriana L described in this work offer
possibilities to generate hairy root cultures for in vitro propagation of plant viruses.
Keywords:
Agrobacterium rhizogenes; antioxidative enzymes; Impatiens walleriana L.Source:
Genetika, 2015, 47, 1, 71-84Funding / projects:
- Development and application of biotechnological methods for obtaining healthy plants of ornamental species (RS-MESTD-Technological Development (TD or TR)-31019)
DOI: 10.2298/GENSR1501071M
ISSN: 0534-0012