Targeting the superoxide/nitric oxide ratio by L arginine and SOD mimic in rat diabetic skin
2016
Аутори:
Janković, AleksandraFerreri, Carla
Filipović, Miloš
Ivanović-Burmazović, Ivana
Stančić, Ana
Otašević, Vesna
Korać, Aleksandra
Buzadžić, Biljana
Korać, Bato
Тип документа:
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Setting the correct ratio of superoxide anion (O2•-) and nitric oxide (•NO) radicals seems to be crucial in restoring disrupted redox signaling in diabetic skin and improvement of •NO physiological action for prevention and treatment of skin injuries in diabetes. In this study we examined the effects of L-arginine and manganese(II)-pentaazamacrocyclic superoxide dismutase (SOD) mimic - M40403 in diabetic rat skin. Following induction of diabetes by alloxan (blood glucose level ≥12 mMol l -1) non-diabetic and diabetic male Mill Hill hybrid hooded rats were divided into three subgroups: (i) control, and receiving: (ii) L-arginine, (iii) M40403. Treatment of diabetic animals started after diabetes induction and lasted for 7 days. Compared to control, lower cutaneous immuno-expression of endothelial NO synthase (eNOS), heme oxygenase 1 (HO1), manganese SOD (MnSOD) and glutathione peroxidase (GSH-Px), in parallel with increased NFE2-related factor 2 (Nrf2) and nitrotyrosine levels characterized diabetic skin. L-arginine and M40403 treatments normalized alloxan-induced increase in nitrotyrosine. This was accompanied by the improvement/restitution of eNOS and HO1 or MnSOD and GSH-Px protein expression levels in diabetic skin following L-arginine, i.e. SOD mimic treatments, respectively. The results indicate that L-arginine and M40403 stabilize redox balance in diabetic skin and suggest the underlying molecular mechanisms. Restitution of skin redox balance by L-arginine and M40403 may represent an effective strategy to ameliorate therapy of diabetic skin.
Напомена:
This is an Accepted Manuscript version of the following article, accepted for publication in Free Radical Research. Jankovic A, Ferreri C, Filipovic M, Ivanovic-Burmazovic I, Stancic A, Otasevic V, Korac A, Buzadzic B, Korac B. Targeting the superoxide/nitric oxide ratio by L-arginine and SOD mimic in diabetic rat skin. Free Radic Res. 2016 Nov;50(sup1):S51-S63. It is deposited under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License http://creativecommons.org/licenses/by-nc-nd/4.0/, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
Кључне речи:
Diabetes; SOD mimic; nitric oxide; redox balance; skin; superoxideИзвор:
Free Radical Research, 2016, 50, Sup1, S51-S63Финансирање / пројекти:
- European Cooperation in Science and Research COST Action CM1201
- Бело или/и мрко: значај масног ткива у одржању укупне редокс зависне метаболичке контроле у физиолошким адаптацијама и метаболичким поремећајима (RS-MESTD-Basic Research (BR or ON)-173055)
DOI: 10.1080/10715762.2016.1232483
ISSN: 1071-5762
PubMed: 27593239