Changes in fractal dimension and lacunarity as early markers of UV-induced apoptosis
2012
Аутори:
Pantic, IgorHarhaji-Trajković, Ljubica
Pantović, Aleksandar
Milošević, Nebojša
Trajković, Vladimir
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2012 Elsevier Ltd.
Метаподаци
Приказ свих података о документуАпстракт:
The aim of our study was to employ fractal analysis for evaluation of ultrastructural changes during early stages of apoptosis. Apoptosis was induced in U251 human glioma cell line by exposure to UVB light. The cells were visualized by optical phase-contrast microscopy and photographed before the UV treatment, immediately after the treatment, as well as at 30 min intervals during 5h observation period. For each of the 32 cells analyzed, cellular and nuclear fractal dimension, as well as nuclear lacunarity, were determined at each time point. Our data demonstrate that cellular ultrastructural complexity determined by fractal dimension and lacunarity significantly decreases after the UV irradiation, with the nuclear lacunarity being a particularly sensitive parameter in detecting early apoptosis. Importantly, fractal analysis was able to detect cellular apoptotic changes earlier than conventional flow cytometric analysis of phosphatidylserine exposure, DNA fragmentation and cell membrane permeabilization. These results indicate that fractal analysis might be a powerful and affordable method for non-invasive early identification of apoptosis in cell cultures.
Кључне речи:
Apoptosis; Fractal analysis; Optical microscopy; UVИзвор:
Journal of Theoretical Biology, 2012, 303, 87-92Финансирање / пројекти:
- Оптичко микроскопска, имуноморфолошка, молекуларно-биолошка и генетска испитивања малигних и немалигних болести бубрега (RS-MESTD-Basic Research (BR or ON)-175059)
- Физичке основе примене неравнотежних плазми у нанотехнологијама и третману материјала (RS-MESTD-MPN2006-2010-141025)
- Структурнe и магнетне особине наночестичних и поликристалних оксида ретких земаља и 3d метала. (RS-MESTD-MPN2006-2010-141027)
DOI: 10.1016/j.jtbi.2012.03.013
ISSN: 1095-8541
PubMed: 22763132