Sesquiterpene lactone production in transformed chicory
2013
Аутори:
Bogdanović, MilicaTodorović, Slađana
Banjanac, Tijana
Dragićević, Milan
Verstappen, Francel
Bouwmeester, Harro
Simonović, Ana
Остала ауторства
Vinterhalter, DraganТип документа:
Конференцијски прилог (Објављена верзија)
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© 2013 the Serbian Biological Society
Метаподаци
Приказ свих података о документуАпстракт:
Chicory (Cichorium intybus L.) is a plant valued for its medicinal and culinary properties. C. intybus owes its healing effect, at least in part, to a group of secondary metabolites known as sesquiterpene lactones. These compounds are shown to have anti-feedant, anti-fungal, anti-bacterial, anti-protozoan, schistomicidal, molluscicidal and anti-cancer effects. Since they are present in relatively small amounts in the plant, there has been ongoing interest in ways to improve the production and yield of these compounds. One way of achieving this goal is Agrobacterium-mediated transformation, which is known to boost secondary metabolism. Chicory is readily transformed with A. rhizogenes to produce hairy-roots, from which whole plants can be regenerated. Several clones previously obtained through A. rhizogenes A4 transformation were analyzed by targeted LC-MS for the content of 8-deoxylactucin, lactucin, dihydrolactucin and lactupicrin. Three clones and one untransformed plant were chosen for the analysis, based on growth, biomass production and initial screening for these metabolites. Both leaves and roots of in vitro grown plants were analysed, in two growth phases: non-flowering rosette and flowering elongated plant. It was found that the selected clones indeed produce more sesquiterpene lactones than the control plants, and that this was dependent on the plant organ and growth phase. Roots in general contain more of these compounds than leaves, and roots of flowering clones have higher content of all target sesquiterpene lactones than control plants. Since these plants were transformed with the native Ri plasmid (pRiA4-GUS), expression of bacterial genes - rolA, rolB and rolC, which are known to affect secondary metabolism, was quantified, in an attempt to correlate elevated compound content with the rol genes’ transcript levels. Expression was highest for the rolC, and barely measurable for rolB. Higher transcript level of these genes was detected in roots, and all three clones had high expression of rolC in the roots of flowering plants, which coincides with sesquiterpene lactones presence.
Кључне речи:
chicory; sesquiterpene lactonesФинансирање / пројекти:
- Физиолошка, хемијска и молекуларна анализа диверзитета одабраних ретких и угрожених биљних врста у циљу еx ситу заштите и продукције биолошки активних једињења (RS-MESTD-Basic Research (BR or ON)-173024)
- Plant Terpenoids for Human Health: a chemical and genomic approach to identify and produce bioactive compounds (EU-FP7-227448)
У:
- Vinterhalter D, editor. Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade. Belgrade: Serbian Plant Physiology Society; 2013. p. 93-4.