Soy phytoestrogen daidzein upregulates ER and p53 in the uterus of middle-aged rat
2015
Autori:
Jarić, IvanaŽivanović, Jasmina
Miler, Marko
Ajdžanović, Vladimir
Trifunović, Svetlana
Nestorović, Nataša
Milošević, Verica
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
,
© 2015 by the Institut national de la recherche agronomique (INRA)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Daidzein (DAI) is bioactive, non-nutritive food ingredient of the traditional East Asian diet and appears to be thoroughly discussed as an alternative to classical hormone replacement therapies. Currently, there is still a lack of information on the potential risks of its’ therapeutic usage. In order to obtain further data useful for estimation of the safety profile of DAI, we investigated the potential of DAI for improvement of uteri function in middle-aged female rats. Also, we investigated the expression of steroid receptors (estrogen – ER, and progesterone – PR) as well as tumor suppressor p53 expression in the uterus of the same animal model.
Middle-aged (13-months-old) female Wistar rats subcutaneously received 35 mg/kg of DAI or the vehicle (olive oil and ethanol, 9:1), daily for 4 weeks. Changes in the structure and function of uteri were analyzed using the histological, immunofluorescence, Western blot and qPCR methods.
DAI did not change uterine weight or histomorphometric characteristics of uterus. Furtherly, the expression of ER and PR mRNA was not affected, while the expression of ER mRNA was increased. The expression level of phospho-p53 was decreased, while p53 was increased after DAI treatment, compared to the control group.
These results suggest that changes in ER and p53 expression/activity in the response to DAI may be an important mechanism to protect endometrial cells from proliferation. Therefore, DAI exhibited beneficial effects on the uterus of middle-aged female rats in the conducted study, figuring as the compound with a promising safety profile, therefore justifying the further investigations pertinent to its efficacy in alleviation of postmenopausal discomforts.
Finansiranje / projekti:
- Odgovor neuroendokrinog sistema pacova na odabrane biljne ekstrakte, fitoestrogene, steroidne i peptidne hormone (RS-MESTD-Basic Research (BR or ON)-173009)
U:
- 7th International Conference on Polyphenols and Health ICPH2015; 2015 Oct 27-30; Tours, France. Institut national de la recherche agronomique (INRA); 2015. p. 248.