Morphometric analysis of pituitary gonadotropic cells after administration of nandrolone decanoate and swimming in adult rats
2019
Аутори:
Sretenović, JasminaŽivković, Vladimir
Srejović, Ivan
Ajdžanović, Vladimir
Milošević, Verica
Milosavljević, Zoran
Jakovljević, Vladimir
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2019 by the Serbian Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
The study included 32 Wistar albino male rats, 10 weeks old, divided into 4 groups: control (T-N-), nandrolone (T-N+), swimming (T+N-) and swimming plus nandrolone (T+N+) groups. The T+ positive groups swam for 4 weeks, 1 hour/day, 5 days/week. The N+ positive groups received nandrolone decanoate (20 mg/kg b.w.) once per week, subcutaneously. Five micrometer thick sections were immunohistochemically stained, while LH and FSH cells were exposed to the morphometric analysis. Circulating level of LH, FSH and testosterone were measured. Body weight did not significantly decrease in all experimental groups compared to control. Absolute pituitary weights were decreased in T-N+ and increased in T+ positive groups. Relative pituitary weights were increased in all experimental groups compared to control. Volume density of LH cells was decreased for 48% in T-N+, for 22% in T+N- and for 35% in T+N+ group. Number of LH cells on mm2 was decreased for 36% in T-N+, for 3% in T+N- and for 27% in T+N+ group. Volume densities of FSH cells were decreased for 39% in T-N+, for 5.5% in T+N- and for 30% in T+N+ compared to control. Number of FSH cells was decreased for 13% in T-N+, for 1% and 15% in T+N+ group compared to control. Serum levels of LH were decreased in N+ positive groups, while FSH level was increased in T+N- group. Serum level of testosterone was increased in all experimental groups compared to control value. Nandrolone alone or combined with swimming decreased morphometric parameters of gonadotropic cells.
Финансирање / пројекти:
- Одговор неуроендокриног система пацова на одабране биљне екстракте, фитоестрогене, стероидне и пептидне хормоне (RS-MESTD-Basic Research (BR or ON)-173009)
У:
- Final program and abstract book: Third Joint Meeting of National Physiological Societies: "Health Risk, Nutrition and Dietary Supplements: Oxidative Stress and Polyphenols in the Heart of Serbian Wineries"; 2019 Jun 20-22; Topola, Serbia. Belgrade: Serbian Physiology Society; 2019. p. 32.