Genistein stimulates trabecular bone structure in orchidectomized middle aged male rats
2016
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Аутори:
Filipović, BrankoŠošić-Jurjević, Branka
Ajdžanović, Vladimir
Ristić, Nataša
Trifunović, Svetlana
Živanović, Jasmina
Pendovski, Lazo
Milošević, Verica
Остала ауторства
Pendovski, LazoPopovska-Perčinić, Florina
Dovenska, Monika
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2016 by the Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine
Метаподаци
Приказ свих података о документуАпстракт:
Introduction: The soybean isoflavones with estrogenic activity are used as natural substitute for hormone replacement therapy for prevention and treatment of postmenopausal osteoporosis. In recent years, more attention has focused on role of estrogens on bone metabolism in men. Therefore, the aim of this study was to investigate the effect of isoflavone genistein on the trabecular bone in orchidectomized (Orx) middle-aged rats as animal model of male osteoporosis.
Material and Methods: Sixteen-month-old Wistar rats were divided into Orx, and a sham-operated (SO) group. Two weeks after gonadectomy, subcutaneous injections of genistein (30 mg/kg b.w./day) were administered for 3 weeks. The SO and one Orx group received sterile olive oil. The animals were killed 24 h after last injection. After fixation and decalcification the proximal tibias were stained by an Azan method. An lmageJ public domain image processing program was used for histomorphometric measurements in the proximal tibial metaphysis and calculate cancellous bone area (B.Ar), trabecular thickness (Tb.Th), trabecular number (Tb.N) and trabecular separation (Tb.Sp). Serum samples were analyzed for osteocalcin (OC) levels, and urine samples for calcium (Ca2+) levels. The data were assessed for normal distribution by the Kolmogorov Smirnov test, followed by one-way analysis of variance (ANOVA).
Results: Analysis of trabecular microarchitecture of proximal tibia in the Orx rats showed a
significant decreases of B.Ar, Tb.Th and Tb.N whereas Tb.Sp was significantly increased compared to SO. After Orx, serum OC and urinary Ca2+ concentration significantly increased in comparison with SO. Genistein treatment in Orx rats significantly enhanced B.Ar, Tb.Th and Tb.N by 115%, 26% and 34% (p<0.05) respectively; but Tb.Sp was 38% lower (p<0.05) when compared to the Orx. Serum OC was 31% lower (p<0.05) after genistein administration compared to values for the Orx rats. Urinary Ca2+ concentration was lower than in the Orx by 68% (p<0.05).
Conclusion: These results suggest that chronic genistein treatment increase trabecular bone mass and decrease bone turnover in the rat model of male osteoporosis.
Кључне речи:
genistein; male rats; orchidectomy; trabecular bone; bone histomorphometryФинансирање / пројекти:
- Одговор неуроендокриног система пацова на одабране биљне екстракте, фитоестрогене, стероидне и пептидне хормоне (RS-MESTD-Basic Research (BR or ON)-173009)
У:
- Pendovski L, Popovska-Perčinić F, Dovenska M, editors. Proceedings: Days of Veterinary Medicine 2016: 7th International Scientific Meeting; 2016 Sep 22-24; Struga, Republic of Macedoina. Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine; 2016. 128-9.