B chromosome inheritance in Apodemus flavicollis
2023
Аутори:
Rajičić, MarijaMiljević, Milan
Bajić, Branka
Budinski, Ivana
Rončević, Aleksa
Vujošević, Mladen
Blagojević, Jelena
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2023 by the Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia
Метаподаци
Приказ свих података о документуАпстракт:
B chromosomes (Bs) are additional elements to the standard karyotype that are not required for
normal growth and development. Bs are predominantly derived from chromosomes of the
standard chromosome set. In some species, these supernumerary chromosomes are passed on to
the next generation more frequently than would be expected under Mendel's law of segregation.
Although the molecular mechanism is unknown, this chromosome drive can occur during
premeiotic, meiotic, or postmeiotic division. Inheritance of B chromosomes across generations is
unknown in many species, including Apodemus flavicollis. The standard karyotype of this
species (2n=48) may contain up to eight B chromosomes, but animals with 1B are most
common. Animals with Bs are present in all studied populations of A. flavicollis in Serbia with
different frequencies (0.10-0.64).
Here we studied the transmission rate of B chromosomes in captive-bred pairs of A. flavicollis.
Juveniles were selected for sex and Bs markers, all of which were determined by PCR. In
addition, primary cell cultures and chromosome preparations were performed for animals with
positive B markers to select animals with 1B. In this way, we were able to form two
combinations of breeding pairs: four pairs with 1B females and 0B males and three pairs with 0B
females and 1B males.
In the pairs where the female had 1B and the male 0B, we obtained a total of 20 offspring, 14 of
which were carriers of the B chromosome (13 with 1B and one animal was a mosaic with 0-2Bs
in the cells). In pairs where males were carriers of 1B and females of 0B, we obtained 10
offspring, of which five animals were carriers of the B chromosome (4 with 1B and one animal
was a mosaic with 0-1B).
In our previous studies of more than forty natural populations, the frequencies of animals with
Bs were the same in males and females. Also, previous studies of meiotic segregation in males
showed that there was no accumulation of Bs, suggesting a general lack of meiotic drive. Herein,
for the first time, we found deviations from Mendel's law of segregation in the transmission of
Bs by the female sex. Our preliminary results suggest the possible presence of a meiotic drive in
the female sex of A. flavicollis, in which ¾ of the offspring inherit B from the mother, whereas in
the male sex B is inherited according to Mendel's law of segregation. However, the small number
of crossed pairs of animals and the small number of litters in the present study indicate the need
to continue this experiment to address the issue of Bs transmission across generations in A.
flavicollis
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- Abstracts: International conference: Chromosome 2023; 2023 Sep 5-10; Novosibirsk, Russia. Novosibirsk: Institute of Molecular and Cellular Biology SB RAS; 2023. p. 42.