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Novel aryl hydrocarbon receptor modulator promotes immunosupressive immune response by stimulating T regulatory cells in the gut
dc.contributor | Dobrijević, Zorana | |
dc.creator | Jonić, Natalija | |
dc.creator | Chatzigiannis, Christos M. | |
dc.creator | Koprivica, Ivan | |
dc.creator | Marinho, Sergio | |
dc.creator | Moura-Alves, Pedro | |
dc.creator | Pavić, Aleksandar | |
dc.creator | Otašević, Vesna | |
dc.creator | Pejnović, Nada | |
dc.creator | Tzakos, Andreas | |
dc.creator | Stojanović, Ivana D. | |
dc.date.accessioned | 2023-11-10T12:55:43Z | |
dc.date.available | 2023-11-10T12:55:43Z | |
dc.date.issued | 2023 | |
dc.identifier.issn | 2787-2947 | |
dc.identifier.uri | http://radar.ibiss.bg.ac.rs/handle/123456789/6295 | |
dc.description.abstract | Introduction: The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor which is highly expressed in mucosal tissues - by epithelial cells and immune cells such as Th17 CD4+ and T regulatory cells (Treg). Besides its function of clearing environmental pollutants from the body, it was also revealed that AhR has immunoregulatory effects, thus becoming a potential therapeutic target for modulating the immune response. For that purpose we tested a novel synthetic AhR modulator under the code name C43. Methods: CYP1A1 (downstream effector of AhR) activation was tested by the EROD assay. Sort-purified CD4+ cells from mesenteric lymph nodes (MLN) were treated with C43 for 24 h. Zebrafish embryos were used to test the toxicity of C43. Male C57BL/6 mice orally received C43 (10 mg/kg) for 5 consecutive days, after which MLN were harvested. Phenotype and function of the cells were analyzed by flow cytometry. Results: C43 showed mild AhR agonistic activity. After treating the sort-purified CD4+ cells with C43, there was a shift in the Th17/Treg ratio in favour of the latter. C43 showed no signs of toxicity when tested on zebrafish embryos. MLN cells from mice that received C43 revealed a shift in the Th1/Treg ratio in favour of Tregs, with a documented rise of the portion of Tregs that expressed CYP1A1 in comparison with the control group of mice. Conclusion: C43 can modulate the immune response through the intestine by promoting the immunosuppressive Treg population. | sr |
dc.language.iso | en | sr |
dc.publisher | Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS// | sr |
dc.rights | openAccess | sr |
dc.source | Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia | sr |
dc.subject | AhR | sr |
dc.subject | immunomodulation | sr |
dc.subject | gut immunity | sr |
dc.subject | Treg | sr |
dc.subject | CYP1A1 | sr |
dc.title | Novel aryl hydrocarbon receptor modulator promotes immunosupressive immune response by stimulating T regulatory cells in the gut | sr |
dc.type | conferenceObject | sr |
dc.rights.license | ARR | sr |
dc.rights.holder | © 2023 by the Institute of Molecular Genetics and Genetic Engineering, University of Belgrade | sr |
dc.description.other | Dobrijević Z, editor. Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia. Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade ; 2023. p. 38. (Trends in Molecular Biology; Special Issue). | sr |
dc.citation.spage | 38 | |
dc.type.version | publishedVersion | sr |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/15290/Natalija_Combos2_abstract.pdf | |
dc.citation.rank | M64 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_6295 |