Adjuvant-free animal model for studying CNS autoimmunity
2022
Тип документа:
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Multiple sclerosis is a chronic inflammatory, demyelinating, and neurodegenerative
disorder of the central nervous system. More than 2.5 million people suffer from this
disease worldwide. It is assumed that the autoimmune response to myelin antigens in the
CNS is the main cause of the disease. Experimental autoimmune encephalomyelitis (EAE)
is a widely used animal model for studying MS. However, EAEmodels resemble only
particular aspects of the MS pathogenesis. EAE is classically induced with the CNS
antigens emulsified in complete Freund’s adjuvant (CFA). CFA consist of paraffin oil
supplemented with Mycobacterium, and its application potentiates innate immune
response, prolongs the presence and effective transport of antigen in the lymphatic system.
However, CFA has a confounding influence on the results and the translational capacity as
a multiple sclerosis model. Our group has successfully excluded CFA from immunization
regime. In a recent study, we compared clinical, histological, cellular and molecular
properties between spinal cord homogenate (SCH) and SCH+CFA immunized Dark
Agouti rats. We have observed higher clinical score in rats without CFA and greater
number of immune cell infiltrates at the peak of EAE in the same animals. Further,
stronger myelin basic protein-specific T cell immune response is evokedin the draining
lymph nodes of CFA-free compared to CFA immunized rats. In the CNS, high abundance
of CD8+T cells is detected at the onset of disease. Also, enrichment in CD8+ and CD4+
macrophageswas observed in the CNS during EAE. Therefore, CFA-free EAE is a reliable
model for studying CNS autoimmunity.
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- Spasojević I, editor. Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia. Belgrade: Faculty of Chemistry; 2022. p. 33.