The effect of a gallic acid derivative on encephalitogenic cells
2022
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Authors:
Stegnjaić, GoranLazarević, Milica
Tsiailanis, Antonios D
Antoniou, Thomas
Gkalpinos, Vasileios K
Nikolovski, Neda
Stanisavljević, Suzana
Dimitrijević, Mirjana
Momčilović, Miljana
Miljković, Đorđe
Tzakos, Andreas G
Jevtić, Bojan
Contributors
Spasojević, IvanDocument Type:
Conference object (Published version)
,
© 2022 by the Faculty of Chemistry, University of Belgrade
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Show full item recordAbstract:
This study aimed to evaluate the effects of a synthetic gallic acid (GA) derivative in the
central nervous system (CNS) autoimmunity, i.e. in experimental autoimmune
encephalomyelitis (EAE), an animal model of multiple sclerosis. EAE was induced in DA
rats by injection of autologous spinal cord homogenate, with a gallic acid derivative being
applied subcutaneously (20 mg/kg, day 7-22 post-immunization). GA derivative
ameliorated EAE. Cells from lymph nodes draining the site of immunization (DLNC),
isolated in the inductive phase of the disease, and spinal cord immune cells (SCIC),
isolated at the peak of disease, were exposed to GA derivative in vitro. Encephalitogenic
cytokines, interferon (IFN)-γ and interleukin (IL)-17, were decreased in SCIC and DLNC
under the influence of GA derivative. The proportion of IL-17-producing CD4+ T cells was
reduced in SCIC (flow cytometry). Treatment of microglial BV2 cells with GA derivative
led to inhibition of NO, IL-6, and tumor necrosis factor release. These results imply that
the synthesized GA derivative is a potent immunomodulator, able to ameliorate EAE. Its
effects on the CNS autoimmunity are related to the inhibition of encephalitogenic T cells
and macrophage/microglia activity in our study.
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- the Hellenic Foundation for Research and Innovation (HFRI) (PROTECT, project no.: 991)
In:
- Spasojević I, editor. Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia. Belgrade: Faculty of Chemistry; 2022. p. 140.