Prikaz osnovnih podataka o dokumentu
Distinct clinical outcomes of Complete Freund’s adjuvant-free experimental autoimmune encephalomyelitis induced in DA rats
dc.contributor | Kanazir, Selma | |
dc.contributor | Savić, Danijela | |
dc.creator | Lazarević, Milica | |
dc.creator | Stegnjaić, Goran | |
dc.creator | Jevtić, Bojan | |
dc.creator | Stanisavljević, Suzana | |
dc.creator | Nikolovski, Neda | |
dc.creator | Momčilović, Miljana | |
dc.creator | Dimitrijević, Mirjana | |
dc.creator | Miljković, Đorđe | |
dc.date.accessioned | 2023-11-10T13:23:39Z | |
dc.date.available | 2023-11-10T13:23:39Z | |
dc.date.issued | 2023 | |
dc.identifier.isbn | 978-86-917255-4-9 | |
dc.identifier.uri | http://radar.ibiss.bg.ac.rs/handle/123456789/6300 | |
dc.description.abstract | Experimental autoimmune encephalomyelitis (EAE) is commonly induced with central nervous system antigens mixed with complete Freund’s adjuvant (CFA). This adjuvant has a confounding influence on the translational capacity of EAE as multiple sclerosis (MS) model. Thus, we developed a novel subtype of EAE induced in Dark Agouti (DA) rats with spinal cord homogenate (SCH) without CFA and characterized it as a reliable MS model. Despite genetic homogeneity of experimental animals and controlled environmental conditions, we observed variations in EAE clinical course in SCH-immunized DA rats and four clinical groups were identified: lethal, severe, moderate, and mild. Immune cells of spinal cord, small intestine lamina propria and lymph nodes draining the site of immunization were compared between moderate and severe group. Higher numbers of CD4+ T cells, regulatory T cells (Treg), helper T cells type 1 (Th1) and 17 (Th17), and B cells were detected in the spinal cords of severe group. Also, higher levels of interferon (IFN)-γ and interleukin (IL)-6 and an increased proportion of Th1 and Th17 cells were detected in the lamina propria of the severe group. Aminoguanidine – an inducible nitric oxide synthase inhibitor that was applied to the rats during the effector phase of the disease ameliorated EAE and imposed a shift of clinical outcomes towards milder variants. Our results suggest that different clinical outcomes in DA rats come as a consequence of variability in the strength of the effector mechanisms exerted within the CNS. The study of the underlying mechanisms for the observed variability is necessary. | sr |
dc.language.iso | en | sr |
dc.publisher | Belgrade: Serbian Neuroscience Society | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS// | sr |
dc.rights | openAccess | sr |
dc.source | Book of abstracts: 8th Congress of Serbian Neuroscience Society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia | sr |
dc.title | Distinct clinical outcomes of Complete Freund’s adjuvant-free experimental autoimmune encephalomyelitis induced in DA rats | sr |
dc.type | conferenceObject | sr |
dc.rights.license | ARR | sr |
dc.rights.holder | © 2023 by Serbian Neuroscience Society and associates. | sr |
dc.description.other | Kanazir S, Savić D, editors. Book of abstracts: 8th Congress of Serbian Neuroscience Society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. Belgrade: Serbian Neuroscience Society; 2023. p. 42. | sr |
dc.citation.spage | 42 | |
dc.type.version | publishedVersion | sr |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/15846/bitstream_15846.pdf | |
dc.citation.rank | M64 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_6300 |