Catechins as Potential Epigenetic Modulators
2018
Аутори:
Jakubek, PatrycjaBaranowska, Monika
Rajić, Jovana
Vidaković, Melita
Bartoszek, Agnieszka
Namiesnik, Jacek
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2018 Silverchair Publisher
Метаподаци
Приказ свих података о документуАпстракт:
Objectives: Year by year, a growing public interest in relationship between diet and ageing can be observed. In particular, this refers to antioxidants, a group of compounds present in food and beverages that are expected to have beneficial health effects. This group includes catechins (favan-3-ols), which are known to play a role in modulation of cellular redox homeostasis and various signaling pathways.
Our previous results have indicated that in HT29 cell line, changes in expression of some genes associated with oxidative stress response are dependent on concentration of catechins. In the subsequent experiments,, we aim at examining the methylation profiles in the promoter areas of up-regulated and down-regulated genes. The objective of the research is to find out whether the observed dose dependence is caused by epigenetic modulation of gene expression.
Methods: The investigation of the impact of different catechins on the expression of genes involved in redox homeostasis was conducted using Oxidative Stress and Antioxidant Defense PCR Array from Qiagen, while DNA methylation is examined using Methylation-Specific PCR (MS-PCR) and Methylation-Sensitive High Resolution Melting (MS-HRM) methods.
Results: A significant up-regulation of 3 genes (ALB, CCL5, HSPA1A) out of 84 included into antioxidant response gene array was observed after treatment of HT29 cell line with investigated catechins at 1 µM concentration. No such up-regulation was seen at 10 µM catechins. Moreover, at this higher dose (-)-epigallocatechin caused down-regulation of SRXN1 gene within the examined gene panel.
Conclusion: Our methylation analysis included two antioxidant response genes, SRXN1 and HSPA1A, whose expression due to its promoter methylation is already documented. Our analysis of the SRXN1 gene regulatory region revealed presence of 706 bp long CpG island that encompasses promotor region and transcription start site, hence conducive to epigenetic silencing via DNA methylation. The impact of DNA methylation on gene expression in control and catechin-treated HT29 cells is in progress.
Финансирање / пројекти:
- program “Maestro 6” (application no: 2014/14/A/ST4/00640)
- COST Action CA16112 “NutRedOx: Personalized Nutrition in aging society: redox control of major age-related diseases
У:
- Selected Abstracts from the 3rd European Summer School on Nutrigenomics; 2018 Jun 25-29; Jesi, Italy. Karger Publishers; 2018. p. 8. (Lifestyle Genomics; Vol. 11; No. 1).