DNA Methylation Changes Induced by Redox-Active Compounds—Choosing the Right PCR-Based Method
2019
Аутори:
Jakubek, PatrycjaRajić, Jovana
Baranowska, Monika
Vidaković, Melita
Bartoszek, Agnieszka
Namiesnik, Jacek
Остала ауторства
Diederich, MarcТип документа:
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
The impact of catechins on the expression profile of redox-related genes in HT29 cell line has been studied recently by our group using Oxidative Stress RT2 Profiler PCR Array. Within the examined panel of 84 genes, the down-regulation of SRXN1 gene was unique among other-regulated genes. We hypothesized that the observed down-regulation resulted from DNA methylation and have exploited this observation to choose the proper strategy to monitor the changes in DNA methylation patterns incurred by dietary antioxidants. The current study verified two PCR-based approaches.
Кључне речи:
catechins; DNA methylation; methylation-specific PCR; methylation-sensitive highresolution melting;; redox homeostasis; epigeneticsФинансирање / пројекти:
- Programme „MAESTRO 6”, application no 2014/14/A/ST4/00640, financed by National Science Centre, Poland
- Сигнални молекули у дијабетесу: идентификација потенцијалних биолошких маркера укључених у модификацију и интеграцију сигналних путева у циљу предикције и интервенције у дијабетесу (RS-MESTD-Basic Research (BR or ON)-173020)
У:
- Diederich M, editor. Marc. CA16112—Luxemburg 2019 meeting; 2019 Mar 25-27; Luxemburg, Luxemburg. Basel: MDPI; 2019. p. 20. (Proceedings; Vol. 11; No. 1).