Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro
2018
Аутори:
Trifunović-Momčilov, MilanaMotyka, Vaclav
Dragićević, Ivana
Paunović, Danijela
Marković, Marija
Subotić, Angelina
Остала ауторства
Uzelac, BrankaТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2018 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
Common centaury (Centaurium erythraea Rafn) is a medicinal plant that can inhabit saline soils.
Previous in vitro studies revealed centaury’s tolerance to salinity concerning shoot regeneration
and development. Centaury is known to spontaneously regenerate shoots from root segments
in vitro on hormone-free medium, probably due to endogenous levels of cytokinins (CKs) and
indolyl-3-acetic acid and their balance in roots. In this study we have investigated in vitro shoot
regeneration from root segments, i.e. frequency of shoot regeneration, the average number of regenerated shoots per explant and shoot biomass production in three transformed centaury lines,
bearing AtCKX1 or AtCKX2 genes. Graded concentrations of NaCl (0–200 mM) were added to the
nutrition medium during 8-week cultivation period. As previously shown, selected transformed
centaury lines grown in vitro were characterized by differently reduced bioactive CK contents, as a
consequence of different expression of transgenes. The main goal was to elucidate if changed CK
homeostasis interferes with salt-tolerance and affects shoot regeneration in centaury. The selected AtCKX centaury lines significantly differed from each other in terms of capacity for spontaneous
shoot regeneration and biomass production in vitro. NaCl lowered shoot regeneration in all of
the investigated lines, although they differed in their sensitivity to salt. Both AtCKX2 lines showed
increased sensitivity to NaCl with the highest sensitivity found in one with the highest potential for
spontaneous regeneration (AtCKX2-26). Concentration of 50 mM NaCl enhanced biomass production only in control and line AtCKX1-29. Our results indicate connection between CK homeostasis,
regeneration capacity and tolerance to salinity.
Кључне речи:
centaury; regeneration in vitro; AtCKX; salinity; cytokininФинансирање / пројекти:
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-MESTD-Basic Research (BR or ON)-173015)
- 16-14649S
У:
- Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 33.