Expression of stress-related genes in diapause of European corn borer (Ostrinia nubilalis Hbn.)
2015
Аутори:
Popović, ŽeljkoSubotić, Ana
Nikolić, Tatjana
Radojičić, Ratko
Blagojević, Duško
Grubor-Lajšić, Gordana
Koštál, Vladimír
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2015 Elsevier Inc.
Метаподаци
Приказ свих података о документуАпстракт:
Diapause is a state of arrested development during which insects cope with many external and internal stressful factors. European corn borer, Ostrinia nubilalis, overwinters as a fifth instar freeze-tolerant diapausing larva. In order to explore diapause-linked stress tolerance processes, the expression of selected genes coding for stress related proteins—glutathione S-transferase (Gst), thioredoxin (Trx), glutaredoxin (Grx), ferritin (Fer), metallothionein (Mtn), and heat shock proteins Hsp90, Hsc70, Hsp20.4, and Hsp20.1—was assessed in the fat body of diapause-destined, warm (22 °C) and cold (5 °C) acclimated diapausing larvae using the quantitative real-time PCR. Gene expression was normalised to mRNA transcripts for Actin and Rps03, and relative expression was calculated using non-diapausing larvae as a control group. During the initiation phase of diapause, the abundance of mRNA transcripts of Grx, Hsp90, Hsc70, andHsp20.1 was significantly upregulated, Trx, Fer, Mtn, andHsp20.1 were unchanged, while only Gst was clearly downregulated in comparison to non-diapause control. Later, in the early phase of diapause, the expression of most genes (except Trx and Hsp20.1) was upregulated in warm-acclimated
larvae, while only Trx and Hsp90 were upregulated in cold-acclimated larvae. Furthermore, the relative expression of all genes (except Trx) increased gradually throughout the diapause in cold-acclimated larvae. This result indicates that the half-life of mRNAs is prolonged during diapause at low temperature, which may lead to a gradual accumulation of mRNA transcripts. Our results show that both diapause programming and temperatures affect the expression of stress-related genes in Ostrinia nubilalis.
Кључне речи:
GST; Redoxin; Ferritin; Metallothionein; HSPsИзвор:
Comparative Biochemistry and Physiology, Part B, 2015, 186, 1-7Финансирање / пројекти:
- Ministry of Education, Youth and Sports of the Czech Republic [LH12103]
- International Visegrad Scholarship Fund (Bratislava, Slovakia) [51000354]
- Молекуларни механизми редокс сигналинга у хомеостази, адаптацији и патологији (RS-MESTD-Basic Research (BR or ON)-173014)
DOI: 10.1016/j.cbpb.2015.04.004
ISSN: 1096-4959
PubMed: 25882225