Graphene quantum dots protect SH-SY5Y cells from SNP induced apoptosis by scavenging reactive oxygen and nitrogen species
2019
Аутори:
Bošnjak, MihajloRistić, Biljana
Krunić, Matija
Mirčić, Aleksandar
Zogović, Nevena
Tovilović-Kovačević, Gordana
Paunović, Verica
Trajković, Vladimir
Harhaji-Trajković, Ljubica
Остала ауторства
Saksida, TamaraStanisavljević, Suzana
Miljković, Đorđe
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2019 by the Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade
Метаподаци
Приказ свих података о документуАпстракт:
We here investigated protective potential of nanoparticles graphene quantum dots
(GQD) against neurotoxicity of sodium nitroprusside (SNP), NO-donor and
antihypertensive drug widely used in studies of nitrosative stress-induced
neurotoxicity. GQD prevented SNP-induced apoptosis, caspase activation and
mitochondrial depolarization in SH-SY5Y neuroblastoma cells. GQD decreased SNP
generated nitrite accumulation in supernatants, as well as NO/ONOO- concentrations
in cells and cell-free medium. However, ONOO- and NO scavengers only slightly
suppressed SNP neurotoxicity. Moreover, light exhausted SNP, incapable of producing
NO, was toxic to SH-SY5Y cells, while GQD strongly reduced its neurotoxicity,
suggesting that defensive effect of GQD far exceeded their NO scavenging activity.
FeSO4 increased death of SH-SY5Y cells, while iron chelators decreased toxicity of
iron-containing SNP. GQD neutralized SNP generated reactive oxygen species (ROS)
production, particularly O2•− and •OH in both cells and cell-free condition.
Neurotoxicity of SNP was suppressed in the presence of unspecific antioxidants,
scavengers of •OH and lipid hydroperoxyl radicals, while it was increased with •OH
generating superoxide dismutase (SOD). Intracellular localization of GQD was
confirmed by transmission electron microscopy (TEM), while extensive washing of
cells preincubated with GQD, only partly reduced their protective activity, suggesting
that GQD exerted neuroprotective effect both intra- and extracellularly. Taken together,
these results suggested that GQD protected neuroblastoma cells by neutralizing reactive
nitrogen species (RNS) and ROS, predominantly •OH formed in Fenton reaction
catalyzed by iron derived from SNP. Therefore, GQD might be promising choice for
treatment of ROS/RNS-mediated neurodegenerative diseases.
Кључне речи:
Graphene quantum dots; neuroprotection; SH-SY5Y cells; ROS scavenging; RNS scavengingФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200110 (Универзитет у Београду, Медицински факултет) (RS-MESTD-inst-2020-200110)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200017 (Универзитет у Београду, Институт за нуклеарне науке Винча, Београд-Винча) (RS-MESTD-inst-2020-200017)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-MESTD-inst-2020-200026)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200146 (Универзитет у Београду, Факултет за физичку хемију) (RS-MESTD-inst-2020-200146)
У:
- Saksida T, Stanisavljević S, Miljković Đ, editors. Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia. Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade; Immunological Society of Serbia; 2019. p. 37.