Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats
2015
Аутори:
Ranković, SlavicaPopović, Tamara
Debeljak Martačić, Jasmina
Tomić, Mirko
Ignjatović, Đurđica
Tovilović-Kovačević, Gordana
Glibetić, Marija
Остала ауторства
Korać, BatoStančić, Ana
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2015 by the Serbian Society for Mitochondrial and Free-Radical Physiology
Метаподаци
Приказ свих података о документуАпстракт:
Dietary intake of different food influences fatty acid composition of lipid fractions, especially phospholipids as the main constituents of cell membranes. The polyunsaturated fatty acids (PUFA) intake is more and more associated with the prevention and development of chronic diseases with an inflammatory component. The liver has an important role in the synthesis and metabolism of phospholipids. Liver phospholipids build the structure of the hepatocyte membrane, take part in metabolic activities and repairing after metabolic disturbances. Our aim was to investigate the effects of food enriched with fish flour and/or food enriched with milk powder on fatty acid composition of liver lipids in rats treated for 4 weeks. Male Wistar rats were randomly assigned into three experimental groups (n=10, 375g±5). The control group recieved standard food (Veterinarski zavod, Subotica). Group II as fed a diet with fish flour and III received a diet with milk powder. The animals were sacrificed by decapitation and part of the liver was frozen at –80 °C. The fatty acid composition of liver phospholipids was determined by Gas Chromatography (Shimadzu GC). Our results showed that food enriched with fish flour significantly increased liver phospholipid concentrations of docosahexaenoic acid (22:6), PUFA and n-3 and decreased n-6/n-3 ratio compared to control. Food enriched with milk powder increased arachidonic acid (20:4), MUFA, PUFA, n-6 and n-6/n-3 ratio while decreased eicosapentaenoic acid (20:5), (22:6) and n-3 compared to control. Fish oil/powder is a well known antioxidant. Food enriched with fish flour is more efficient due to decrease of n-6/n-3 ratio (as a risk factor) and our future examinations will address it.
Кључне речи:
polyunsaturated fatty acids (PUFA); fish flour; liver phospholipids; Gas ChromatographyФинансирање / пројекти:
- Биолошки механизми, нутритивни унос и статус полинезасићених масних киселина и фолата: Унапређење исхране у Србији (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41030)
У:
- Korać B, Stančić A, editors. Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia. Belgrade: Serbian Society for Mitochondrial and Free-Radical Physiology; 2015. p. 85.