Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes.
2023
Аутори:
De Oliveira, IzamaraChrysargyris, Antonios
Heleno, Sandrina A.
Carocho, Marcio
Calhelha, Ricardo
Dias, Maria Ines
Petrović, Jovana
Soković, Marina
Petropoulos, Spyridon
Santos-Buelga, Celestino
Tzortzakis, Nikolaos
Barros, Lillian
Тип документа:
Чланак у часопису (Објављена верзија)
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© 2023 Elsevier Ltd.
Метаподаци
Приказ свих података о документуАпстракт:
The present study aims to determine the combined effect of cropping system and irrigation regime on the chemical composition and bioactive properties of lemon balm aerial parts. For this purpose, lemon balm plants were grown under two farming systems (conventional farming vs organic farming) and two irrigation levels (full irrigation vs deficit irrigation) and harvested twice throughout the growing period. The collected aerial parts were subjected to three different methods of extractions, namely infusion, maceration and ultrasound-assisted extraction and the obtained extracts were evaluated in terms of chemical profile and bioactivities. Five organic acids with varied composition among the tested treatments were identified in all the tested samples for both harvests, namely, citric, malic, oxalic, shikimic and quinic acid. Regarding phenolic compounds composition, the most abundant ones were rosmarinic acid, lithospermic acid A isomer I and hydroxylsalvianolic E, especially for the maceration and infusion extraction methods. Full irrigation resulted in lower EC50 values than deficit irrigation only in the treatments of the second harvest, while variable cytotoxic and anti-inflammatory effects were recorded in both harvests. Finally, in most cases the lemon balm extracts has similar or better activity than the positive controls, while the antifungal activity of lemon balm extracts was stronger than the antibacterial effects. In conclusion, the results of the present study showed that the implemented agronomic practices, as well as the extraction protocol may significantly affect the chemical profile and bioactivities of lemon balm extracts, suggesting that both the farming system and the irrigation schedule may improve the quality of the extracts depending on the implemented extraction protocol.
Кључне речи:
Organic cultivation; Deficit irrigation; Bioactive potential; Phenolic compounds; Maceration; Infusion; Ultrasound assisted extraction; Antimicrobial activitiesИзвор:
Food Research International, 2023, 170, 113044-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
DOI: 10.1016/j.foodres.2023.113044
ISSN: 0963-9969
PubMed: 37316092