Age-dependent catalase activity in Empress tree (Paulownia tomentosa Steud.) seeds
2013
Authors:
Milutinović, MilicaŽivković, Suzana
Dragićević, Milan
Cvetković, Jelena
Simonović, Ana
Bogdanović Pristov, Jelena
Todorović, Slađana
Contributors
Vinterhalter, DraganDocument Type:
Conference object (Published version)
,
© 2013 by the Serbian Plant Physiology Society
Metadata
Show full item recordAbstract:
In plants, catalase (CAT) is considered to play a key role in protection and repair systems during seed ageing. The aim of our study was to investigate the effects of light, GA3, KN03 and liquid smoke on germination, CAT activity and CAT isozyme pattern of positive photoblastic Empress tree (Paulownia tomentosa Steud.) seeds of different ages. Freshly harvested Empress tree seeds require long term periods of light for germination. However, the application of KN03 and liquid smoke extract could stimulate germination and overcome the light requirement. During the process of after-ripening in young seeds, their sensitivity to light in creases and a single 5 min pulse of red light is sufficient to induce maximum germination. Over longer seed storage, seeds lose ability to germinate and become less viable. No germination was detected in seeds older than 8 years. We observed a correlation between the level of germination and catalase activity. lts activity is higher in mature seeds then in freshly harvested seeds during light-induced germination. In young seeds, CAT activity is enhanced by exogenous seed germination stimulators application. In very old, non viable seeds, CAT activity is reduced to basal level (CAT activity of dry seeds). Different CAT isozyme patterns were observed in dark- and light germinated seeds.
Funding / projects:
- Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering (RS-MESTD-Basic Research (BR or ON)-173017)
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
In:
- Vinterhalter D, editor. Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade. Belgrade: Serbian Plant Physiology Society; 2013. p. 31.