mTOR signaling pathway changes under the effect of caloric restriction in the cortex of male Wistar rats
2023
Аутори:
Prvulović, MilicaTodorović, Smilja
Milanović, Desanka
Simeunović, Valentina
Vukojević, Anđela
Jović, Milena
Sokanović, Srđan
Mladenović, Aleksandra
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2023 Elsevier Inc.
Метаподаци
Приказ свих података о документуАпстракт:
Although delaying age-related changes is a recognized effect of
caloric restriction (CR), its duration along with a person's age and
sex, can significantly alter its protective potential and even have a
detrimental effect when implemented later in life. One of the
underlying mechanisms of the effect of CR on aging may be through
the mTOR pathway, as its important role in nutrient sensing and
regulation of cellular metabolism is well known. We investigated the
expression of key molecules of this signaling pathway during aging
and under different CR regimens. Male Wistar rats of different ages
(adult, middle-aged, and old) were exposed to CR (60% of AL) to
study the effect of CR at early (EOCR) and at late onset (LOCR). Ad
libitum (AL) fed animals were used to test the effects of aging and as
age-matched controls for the effect of CR. Western blot was used to
analyze the expression of proteins involved in the signaling cascade
and the interaction of mTOR complexes 1 and 2 (mTORC1,
mTORC2) in rat cortex. At 18 months of age, both EOCR and LOCR
led to a decrease in phosphorylation of mTOR S2448, which is
mainly associated with MTORC1, whereas LOCR led to an increase at
24 months of age. Phosphorylation of mTOR S2481 (mainly asso ciated with mTORC2) increased with age, whereas LOCR resulted in
a decrease at both ages. Although both EOCR and LOCR led to a
decrease in phosphorylation of mTOR at 18 months of age, some
effects were lost at 24 months. Changes in the expression of two
different phosphorylated forms of AKT responsible for communica tion between MTORC1 and MTORC2 were inconsistent, suggesting that another kinase may be responsible for mTOR phosphorylation
without affecting AKT.
Кључне речи:
Caloric restriction; mTOR pathway; Cortex; AgingФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- IBRO 11th World Congress of Neuroscience; 2023 Sep 9-13; Granada, Spain. Elsevier; 2023. p. S478. (IBRO neuroscience reports; Vol. 15; Suppl. 1).