Analysis of amplification of microsatellite loci in Iris pumila
2022
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Authors:
Avramov, StevanBanjanac, Tijana
Brkušanin, Miloš
Šiler, Branislav
Miljković, Danijela
Barišić Klisarić, Nataša
Živković, Uroš
Tarasjev, Aleksej
Contributors
Milutinović, MilicaDocument Type:
Conference object (Published version)
,
© 2022 by the Serbian Plant Physiology Society
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Show full item recordAbstract:
Utilization of microsatellite molecular markers was analyzed in Iris pumila and five other species
belonging to the same genus: I. humilis, I. sibirica, I. pseudacorus, I. spuria and I. variegata, which
inhabit the northern part of the Republic of Serbia. We selected 40 EST-SSR markers previously
reported for the genus Iris. Sixteen markers that showed polymorphism were chosen for further
research. To examine the potential of the selected microsatellite loci analysis in the detection of
different I. pumila clones, we selected 18 individuals of this species that belonged to different
genotypes. Optimization of amplification was performed for all 16 microsatellite loci: temperature
program of the PCR protocol and number of amplification cycles were optimized as well as primer
and DNA concentrations used in the reactions. Seven markers that were variable across the ana lyzed individuals were selected. The final analysis was performed using only 5 microsatellite loci
since high-quality amplification for two loci was not possible. No greater deviations in the length
of the fragments from the expected were observed, except for one primer. All microsatellite loci
contained three-nucleotide repeats. I. pumila is tetraploid species and in several plants the presence of four alleles were observed. The observed number of alleles per locus ranged from 3 to 9.
In the results obtained across 18 I. pumila individuals using 5 microsatellite markers, 17 multilocus
genotypes were observed.
Keywords:
Iris pumila; EST-SSR markers; multilocus allelic phenotypeFunding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
In:
- Milutinović M, editor. Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; 2022. p. 147.