The characterization of silymarin and silibinin liposomes
2022
Authors:
Jovanović, AleksandraDinić, Svetlana
Uskoković, Aleksandra
Arambašić Jovanović, Jelena
Grdović, Nevena
Vidaković, Melita
Mihailović, Mirjana
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
The aim of the present study was the characterization of silymarin and silibinin li-
posomes
via
determination of encapsulation efficiency, particle size, polydispersity
index (PDI), zeta potential, mobility, and conductivity, as well as storage stability
during 28 days at 4ºC and stability after UV irradiation. Encapsulation efficiencies
of silymarin and silibinin were 92.05±1.41% and 87.86±2.06%, respectively. Par-
ticle size and PDI of the liposomes with silymarin were changed from 3541.3±62.5
nm to 2677.0±44.2 nm and from 0.346±0.044 to 0.228±0.036, respectively, dur-
ing the 28-day stability study; particle size and PDI of the liposomes with silibinin
were changed from 2074.7±19.4 nm to 2704.0±35.0 nm and from 0.328±0.030
to 0.456±0.026, respectively. The Zeta potential of the silymarin-loaded liposomes
and silibinin-loaded liposomes was changed from -27.0±0.7 mV to -26.4±0.4 mV
and from -29.4±0.6 mV to -29.0±0.4 mV, respectively. Mobility and conductivity
of the liposomes with silymarin were changed from -2.120±0.057 μmcm/Vs to -
2.067±0.028 μmcm/Vs and from 0.017±0.005 mS/cm to 0.009±0.004 mS/cm,
respectively. Mobility and conductivity of the liposomal particles with silibinin
were changed from -2.307±0.053 μmcm/Vs to -2.110±0.033 μmcm/Vs and from
0.018±0.003 mS/cm to 0.060±0.001 mS/cm, respectively. UV irradiation did not
affect particle size and PDI of all liposomes, but it caused a decrease in zeta po-
tential: -23.9±0.8 mV for silymarin and -24.5±0.7 mV for silibinin, in mobility: -
1.874±0.064 μmcm/Vs for silymarin and -1.920±0.057 μmcm/Vs for silibinin, and
in conductivity: 0.014±0.001 mS/cm for silymarin and 0.007±0.003 mS/cm for
silibinin. Overall, the obtained results qualify liposomes to be used as silymarin and
silibinin carriers for application in functional foods and pharmaceutical products.
Keywords:
silymarin; silibinin; liposomes; characterizationSource:
Journal of Engineering and Processing Management, 2022, 14, 2, 40-45Funding / projects:
- Signaling molecules in diabetes: search for potential targets in intrinsic pathways for prediction and intervention in diabetes (RS-MESTD-Basic Research (BR or ON)-173020)