Isolation of microplastics from freshwater Asian clams Corbicula fluminea (Müller, 1774) in the Danube River
2023
Аутори:
Stanković, JelenaPaunović, Momir
Milošević, Đurađ
Lončarević, Davor
Radulović, Katarina
Kolarević, Stoimir
Tomović, Jelena
Raković, Maja
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2023 by the Austrian Committee - International Association for Danube Research
Метаподаци
Приказ свих података о документуАпстракт:
During the Joint Danube Survey 4 in 2019, we investigated plastic debris in Danube River, from Germany to the Black Sea (more than 2,000 km of the river stretch). The main aim of the study was to categorize and to quantify microplastic particles in living systems. Freshwater Asian clams Corbicula fluminea (Müller, 1774) was used as test organism. Individuals were collected from 23 sites using a hand net (ap. 25 cm× 25 cm, mesh size 500 μm). In order to isolate plastic particles, the samples were digested by alkaline method, using a KOH 10% solution and incubation at 65 ⁰C for 12 h. The digested samples were filtrated through a glass microfiber filters, with 0.5 µm mesh size. Collected particles were photographed and categorized based on the size and coloration. Particles were counted manually, photographed using Nikon SMZ 745T Stereomicroscope and measured in program ImageJ. In 216 examined specimens a total of 1,998 microplastic particles were isolated with an average of 5.59 ± 3.71 fibrils and 4.37 ± 2.46 fragments per organism; or 40.77 ± 73.75 fibrils and 25.84 ± 33.17 fragments per g body weight. Dominant microplastic particles were between 0.4 and 0.5 mm in diameter (characterized as medium-sized), with an average length 0.43 mm ± 0.26 in the Danube and 0.49 mm ± 0.26 in the tributaries. In order to confirm chemical composition of isolated microliter, 46 particles of the hard plastic from 14 sampling sites were analyzed using Nicolet iN10 Fourier transform infrared microscope with micro ATR accessory and cooled MCT detector, using 128 scans at resolution of 4 cm1 . Analyses revealed presence of five different types of polymers, with the domination of polyethylene-terephthalate.
Кључне речи:
microplastic; Danube River; Asian clam; micro- ATR-FTIRУ:
- Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. Austrian Committee - International Association for Danube Research; 2023. p. 21.