Ispitivanje uticaja ekstrakta vlašca (Allium schoenoprasum L.) i kvercetina na morfološke i fiziološke karakteristike klijanaca Arabidopsis thaliana

Abstract

Ekstrakt listova vlašca je bogat flavonolima (kemferol, kvercetin i izoramnetin) i flavonol nim glikozidima (kemferol-3-O-glikozid i kvercetin-3-O-glikozid). Ova jedinjenja predstavljaju važnu grupu flanovoida sa širokim spektrom biološke aktivnosti. Cilj ovog istraživanja je da se ispita kako klijanci model biljke A. thaliana reaguju na prisustvo ekstrakta vlašca (koncentracije 100 mg/L) i kvercetina (koncentracije 15.11 mg/L) u hranljivoj podlozi. Dužina hipokotila je iz merena petog, sedmog i desetog dana nakon klijanja semena. Na elongaciju hipokotila značajno utiče prisustvo kvercetina u podlozi. Zahvaljući DAB (3,3´- diaminobenzidin) bojenju detekto vana je akumulacija H2O2 u biljnom tkivu klijanaca A. thaliana starosti 10 dana. Ovaj rezultat je u saglasnosti sa povećanom aktivnošću antioksidativnog enzima peroksidaza kod klijanaca koji su rasli na podlozi sa ekstraktom vlašca. Takođe je određena i aktivnost enzima glutation transferaza, koji se dominantno nalazi u citosolu i učestvuje u procesima detoksikacije biljne ćelije. Opisane promene u aktivnosti antioksidativnih enzima klijanaca model biljke na podlozi sa ekstraktom vlašca, usmeravaju nas ka novim koracima u istraživanju sa mogućnošću razvoja potencijalnih bioherbicida

Chive leaf extract is a rich source of flavonols (kaempferol, quercetin and izorahmentin) and flavonoid glycosides (kaempferol-3-glucoside and quercetin-3-glucoside). These compounds are a very important group of flavonoids that have a broad range of biological activities. The aim of this study is to investigate the response of Arabidopsis thaliana to the presence of chive extract (concentration of 100 mg/L) and quercetin (concentration of 15.11 mg/L) in the growth medium. The length of hypocotyl was measured on the 5th, 7th, and 10th day after the germination of seeds. The presence of quercetin in the growth medium significantly effects the elongation of the hypocotyl. Accumulation of H2O2 in the plant tissue of seedlings was detected by DAB staining (3,3’-diami nobenzidine). These results correspond with increased activity of antioxidative enzyme peroxidase in seedlings that grow on the medium with chive extract. The activity of glutathione transferase was also determined. This enzyme is predominantly located in the cytosol and participates in the process of detoxification of plant cells. The described changes in antioxidative enzyme activities in plant model seedlings on growth medium with chive extract guide us towards new steps in research with the possible development of a potential bioherbicide