Enzymatic component of antioxidative system in succulent plant Tacitus bellus as a response to hemibiotroph Fusarium verticillioides infection in vitro
2022
Аутори:
Cvetić Antić, TijanaJanošević, Dušica
Glamočlija, Jasmina
Mitrović, Aleksandra
Остала ауторства
Milutinović, MilicaТип документа:
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Fungi and plants interact in different ways, creating a scale of associations. Hemibiotrophic
fungi represent the most interesting group, as they use sequential biotrophic and necrotrophic infection strategies. The co-evolution of plant and fungal life-styles has not been well characterized.
We present changes of enzymatic component of antioxidative system in succulent plant Tacitus
bellus that specifically correspond to subsequent phases (spore germination, biotrophic phase,
and necrotrophic phase) of hemibiotroph Fusarium verticillioides infection. T. bellus response to F.
verticillioides spore germination was characterized by transient increase in catalase (CAT), but decrease in superoxide dismutase (SOD) and peroxidase (POD) activity. During biotrophic phase of F.
verticillioides infection, when hyphae spread intercellularly in epidermal and mesophyll tissue, host
antioxidative system was suppressed. The transition from biotrophic to necrotrophic phase (inter
and intracellular colonization and sporulation) triggered the host plant cells to create a highly defensive environment: CAT, SOD and POD activities were significantly stimulated, slowing, or even
currently arresting, colonization of T. bellus mesophyll cells. CAT, showing the most pronounced
activity increase, could be suggested as the main enzyme responsible for slowing the progression
of necrotrophic phase of F. verticillioides growth. However, contrary to host CAT and SOD which
isoenzyme profile didn’t change, new highly acidic POD isoforms replaced the two mildly acidic
isoforms, suggesting their specific role in slowing the progression of infection. Presented results
add to knowledge of events and mechanisms related to hemibiotrophic fungi pathogenicity in
succulent plants grown under high relative humidity, similar to conditions in greenhouse.
Кључне речи:
Tacitus bellus; Fusarium verticillioides; fungal leaf infection; antioxidative enzymesФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-MESTD-inst-2020-200053)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200178 (Универзитет у Београду, Биолошки факултет) (RS-MESTD-inst-2020-200178)
У:
- Milutinović M, editor. Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; 2022. p. 50.