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Nrf2 coordinates redox-metabolic homeostasis required for lipid deposition in rat visceral adipose tissue during the re-acclimation of rats after cold
dc.creator | Budnar Šoškić, Marta | |
dc.creator | Zakić, Tamara | |
dc.creator | Korać, Aleksandra | |
dc.creator | Korać, Bato | |
dc.creator | Janković, Aleksandra | |
dc.date.accessioned | 2024-02-06T14:53:19Z | |
dc.date.available | 2900-01-01 | |
dc.date.issued | 2023 | |
dc.identifier.issn | 0891-5849 | |
dc.identifier.uri | http://radar.ibiss.bg.ac.rs/handle/123456789/6526 | |
dc.description.abstract | Тight control of the lipid-deposition function of white adipose tissue (WAT) defines the overall metabolic homeostasis and entails coordinated antioxidant response. We aimed to reveal temporal changes in the antioxidant defence (AD) that is a prerequisite for lipid metabolism in the visceral WAT over 45 days of re-acclimation to room temperature (RT, 22±1 °C) in rats pre-acclimated to cold (45 days, 4±1 °C). The Nuclear factor erythroid 2–related factor 2 (Nrf2) and its downstream antioxidant targets, as well as key lipogenic enzymes were examined in epididymal WAT (eWAT) and compared to the RT-maintained control. Upregulation of Nrf2 protein expression observed from the 1-12th day of re-acclimation was followed by an increase in the glutathione level (3-7th day) and the protein expression of copper, zinc superoxide dismutase (3-12th day), manganese superoxide dismutase (1-12th day), thioredoxin (1-45th day), and glutathione peroxidase (1-45th day). Such time-dependent changes in AD during re-acclimation coincided with increases in the lipogenic enzymes acetyl-CoA carboxylase and fatty acid synthase that were further supported by elevated glyceraldehyde 3-phosphate dehydrogenase and glucose 6-phosphate dehydrogenase protein levels. Observed redox-metabolic integration in eWAT resulted in the restitution of relative eWAT mass on the 12th day of re-acclimation. It can be concluded that Nrf2-coordinated redox-metabolic changes drive the restitution of eWAT lipid depot during the re-acclimation of rats after cold. These results show a novel role of Nrf2 in regulating redox-metabolic signaling under homeostatic conditions and its relevance in visceral adiposity in different metabolic diseases. | sr |
dc.language.iso | en | sr |
dc.publisher | Elsevier | sr |
dc.relation | info:eu-repo/grantAgreement/ScienceFundRS/Promis/6066747/RS// | sr |
dc.rights | restrictedAccess | sr |
dc.source | Redox Biology Congress 2023; 2023 Jun 6-9; Vienna, Austria | sr |
dc.title | Nrf2 coordinates redox-metabolic homeostasis required for lipid deposition in rat visceral adipose tissue during the re-acclimation of rats after cold | sr |
dc.type | conferenceObject | sr |
dc.rights.license | ARR | sr |
dc.rights.holder | © 2023 Published by Elsevier Inc. | sr |
dc.description.other | Redox Biology Congress 2023; 2023 Jun 6-9; Vienna, Austria. Elsevier; 2023. p. 26. (Free Radical Biology and Medicine; Vol. 201; Suppl. 1). | sr |
dc.identifier.doi | 10.1016/j.freeradbiomed.2023.03.183 | |
dc.identifier.wos | 000994966600085 | |
dc.citation.spage | 26 | |
dc.type.version | publishedVersion | sr |
dc.citation.rank | M34 |