Crocus sativus tepals extract suppresses subcutaneous adipose tissue hypertrophy and improves systemic insulin sensitivity in mice on high-fat diet
2024
Autori:
Bursać, BiljanaBellachioma, Luisa
Gligorovska, Ljupka
Jovanović, Mirna
Teofilović, Ana
Vratarić, Miloš
Vojnović Milutinović, Danijela
Albacete, Alfonso
Martínez-Melgarejo, Purificación A
Morresi, Camilla
Damiani, Elisabetta
Bacchetti, Tiziana
Đorđević, Ana
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2024 International Union of Biochemistry and Molecular Biology.
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Obesity is a pressing problem worldwide for which standard therapeutic strategies have limited effectiveness. The use of natural products seems to be a promising approach to alleviate obesity and its associated complications. The tepals of Crocus sativus plant, usually wasted in saffron production, are an unexplored source of bioactive compounds. Our aim was to elucidate the mechanisms of Crocus sativus (Cr) tepals extract in obesity by investigating its effects on adipocyte differentiation, visceral (VAT) and subcutaneous (SAT) adipose tissue hypertrophy, and lipid metabolism in an animal model of diet-induced obesity. To this end, mouse 3T3-F442A preadipocytes were treated with Cr tepals extract and the expression of adipocyte differentiation genes was determined. Caloric intake, body mass, triglycerides, systemic insulin sensitivity, histology, insulin signaling and lipid metabolism in VAT and SAT were analyzed in mice fed a 60% fat diet for 14 weeks and treated orally with Cr tepals extract during the last 5 weeks of the diet. We demonstrated for the first time that Cr tepals extract inhibits adipocyte differentiation in vitro. The animal model confirmed that oral treatment with Cr tepals extract results in weight loss, improved systemic insulin sensitivity, lower triglycerides, and improved lipid peroxidation. The suppressive effect of Cr tepals extract on adipocyte hypertrophy and inflammation was observed only in SAT, which, together with preserved SAT insulin signaling, most likely contributed to improved systemic insulin sensitivity. Our results suggest the functionality of SAT as a possible target for the treatment of obesity and its complications.
Ključne reči:
adipose tissue; Crocus sativus; insulin sensitivity; lipid metabolism; obesityIzvor:
BioFactors, 2024Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
Povezane informacije:
- Druga verzija
https://radar.ibiss.bg.ac.rs/handle/123456789/6533