Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene
2018
Authors:
Trajković, MilenaJeknić, Zoran
Antonić Reljin, Dragana
Subotić, Angelina
Jevremović, Slađana
Cingel, Aleksandar
Contributors
Uzelac, BrankaDocument Type:
Conference object (Published version)
,
© 2018 by the Serbian Plant Physiology Society
Metadata
Show full item recordAbstract:
Viola cornuta L. ‘Lutea Splendens’ is a perennial ornamental plant with small yellow flowers that
naturally grows in the Pyrenees in Spain and France. To develop novel cultivars with orange and
red flower colors, we performed Agrobacterium tumefaciens (LBA4404)-mediated transformation
with the binary vector pWBVec10a/P35S::Llccs::TNos that harbored capsanthin-capsorubin synthase
(Llccs) gene from Lilium lancifolium under the control of CaMV35S constitutive promoter and
the nopaline synthase (Nos) terminator. Capsanthin-capsorubin synthase catalyzes the conversion
of anteraxanthin and violaxanthin, two yellow ubiquitous 5-6-epoxy-xanthophylls, into capsanthin
and capsorubin, two red xanthophylls, respectively. Starting with hypocotyl explants, we developed
a transformation protocol with 0.3% shoot regeneration efficiency. Histochemical assay for
β-glucuronidase (GUS) activity showed uidA reporter gene expression in all putative Llccs-transgenic
shoots. The presence of Llccs transgene, hygromycin phosphotransferase (hpt) selectable
marker gene and uidA (GUS) reporter gene in all putative Llccs-transgenic lines were confirmed
by PCR analysis. This is the first report on Agrobacterium-mediated genetic transformation of V.
cornuta L. with the aim to introduce desirable traits into this species.
Keywords:
horned pansy; flower color; capsanthin; capsorubin; carotenoid biosynthesisFunding / projects:
- Development and application of biotechnological methods for obtaining healthy plants of ornamental species (RS-MESTD-Technological Development (TD or TR)-31019)
In:
- Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 152.