The effect of O-GlcNAc glycosylation of rat liver nucleoproteins on their acute phase-dependent binding ability to the hormone responsive element of the haptoglobin gene
2004
Аутори:
Grigorov, IlijanaCvetković, Ivana
Milosavljević, Tomislav
Uskoković, Aleksandra
Bogojević, Desanka
Ivanović Matić, Svetlana
Poznanović, Goran
Petrović, Miodrag
Тип документа:
Чланак у часопису (Објављена верзија)
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© by the publisher
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Приказ свих података о документуАпстракт:
We examined whether the transcriptional activation of the rat haptoglobin (Hp) gene during the acute phase (AP) response reflects the O-linked N-acetylglucosamine (O-GlcNAc) status of liver nucleoproteins (NPs) and their binding for the hormone responsive element (HRE). After deglycosylation with N-acetylglucosaininidase of the O-GlcNAc glycoproteins obtained by WGA, affinity chromatography and South-Western analysis, it was observed that only increased HRE binding ability of p64/p70 in control and p51 obtained from turpentine-treated rats can be directly attributed to the presence of O-GlcNAc residues. Therefore, expression of the rat Hp gene could be controlled by this modification of certain trans-acting NPs.
Кључне речи:
O-GlcNAc glycosylation; Trans-acting proteins; DNA binding; Haptoglobin gene; Acute phase responseИзвор:
General Physiology and Biophysics, 2004, 23, 3, 367-374Финансирање / пројекти:
- the Ministry for Science and Technology of Serbia and Montenegro, grant No. 1722