Integration of metabolomics and transcriptomics data reveals the molecular background of the iridoid diversity within the genus Nepeta (fam. Lamiaceae)
2023
Аутори:
Mišić, DanijelaGašić, Uroš
Šiler, Branislav
Dmitrović, Slavica
Skorić, Marijana
Matekalo, Dragana
Nestorović Živković, Jasmina
Banjanac, Tijana
Filipović, Biljana
Milutinović, Milica
Božunović, Jelena
Aničić, Neda
Petrović, Luka
Lukić, Tamara
Todorović, Miloš
Остала ауторства
Dobrivojević, ZoranaТип документа:
Предавање (Објављена верзија)
,
© 2023 by the Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
Метаподаци
Приказ свих података о документуАпстракт:
Introduction: Recent intensive research on the genus Nepeta has resulted in increasingly accumulated
information regarding the biosynthetic pathways and chemical evolution of iridoids; however, several in-
triguing aspects remain yet to be resolved. Our objective was to deeply investigate the molecular back-
ground of the diversity of iridoid compounds within the genus Nepeta, as well as regulative mechanisms
of their biosynthesis.
Methods: Leaves of greenhouse-grown plants were analysed for the composition of iridoid aglycones
(IAs) and iridoid glucosides (IGs) adopting non-targeted (UHPLC/LTQ Orbitrap MS n
, GC/MS) and targeted
(UHPLC/DAD/(±)HESI-MS 2 ) metabolomics approaches. Following RNA-Seq, transcriptomes of phylodi-
verse Nepeta taxa were searched for the presence/absence of iridoid-related biosynthetic genes. Co-ex-
pression patterns (qPCR) of biosynthetic genes and transcription factors were determined following
plants’ exposure to various environmental stresses.
Results: The comparison of metabolite composition among phylodiverse Nepeta taxa provided a sys-
tematic understanding of qualitative and quantitative composition of iridoids in leaves of the selected
Nepeta taxa. Mining of RNA-Seq data in search for iridoid biosynthesis-related genes pointed to signifi-
cant differences between taxa producing both IAs and IGs and those producing only IGs. Comparative
metabolomics and gene co-expression analysis provided new information about mechanisms of regu-
lation of iridoid biosynthesis.
Conclusion: Integration of data from several techniques analysed by different methods resulted in iden-
tifying key genes involved in the regulation of metabolic flux through the iridoid biosynthetic pathway
and offered an explanation why some Nepeta taxa produce only IGs, while others produce both IAs and
IGs. The obtained results pointed to new gene targets for improving iridoid production through biotech-
nology-based approaches.
Кључне речи:
Nepeta; Iridoids; metabolomics; transcriptomics; gene co-expression analysisФинансирање / пројекти:
- NEPETOME - Omics-guided disentangling of the iridoid diversity in the genus Nepeta L. for in planta metabolic engineering towards sustainable exploitation (RS-ScienceFundRS-Ideje-7749433)
У:
- Dobrijević Z, editor. Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia. Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade ; 2023. p. 133. (Trends in Molecular Biology; Special Issue).