Polyethylene glycol (PEG)-induced dehydration alters enzymatic and non-enzymatic components of the antioxidant defense system in Nepeta nervosa Royle ex Bentham.
2023
Аутори:
Nestorović Živković, JasminaAničić, Neda
Matekalo, Dragana
Skorić, Marijana
Filipović, Biljana
Marković, Tijana
Dmitrović, Slavica
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Plants have developed a variety of defense mechanisms that allow them to recognize
and respond to specific stressors by triggering complex signaling networks that cause appropriate
biochemical changes to overcome the stress. In this study, we subjected in vitro grown N. nervosa
plants to PEG-induced dehydration stress for 1 day (1DPT), 3 days (3DPT) and 6 days (6DPT). Our
study investigated antioxidant enzyme activities, including catalase (CAT), peroxidase (POX) and
superoxide dismutase (SOD), unveiling dynamic responses to PEG-induced water stress. CAT levels
increased initially (1DPT) but declined with prolonged treatment; while POX activity significantly
increased at 3DPT and 6DPT; and SOD, particularly the Mn-SOD3 isoform, demonstrated a substantial
increase, emphasizing its role in the enzymatic free-radical scavenging activity. Furthermore,
examination of the phenolic acid content revealed that rosmarinic acid (RA) was the predominant
phenolic compound, followed by chlorogenic acid (CHLA), while ferulic acid (FA) and caffeic acid
(CAFFA) were present in lower concentrations. Notably, PEG-induced dehydration significantly
boosted RA content in N. nervosa plants at 3DPT. This increase highlights the plant’s response to
oxidative stress conditions and its role in non-enzymatic antioxidant defense mechanisms. These
findings significantly contribute to our comprehension of N. nervosa’s adaptive strategies under PEG induced dehydration stress, offering valuable insights into plant stress physiology within industrial
and agricultural contexts.
Кључне речи:
dehydration; polyethylene glycol; rosmarinic acid; antioxidant defense systems; Nepeta nervosa; in vitro culture; abiotic stress; antioxidant enzymesИзвор:
Horticulturae, 2023, 9, 12, 1277-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
DOI: 10.3390/horticulturae9121277
ISSN: 2311-7524