Progress in disentangling the diversity of iridoids within the genus Nepeta: surprising biosynthetic and evolutionary insights
2022
Аутори:
Aničić, NedaMatekalo, Dragana
Skorić, Marijana
Božunović, Jelena
Nestorović Živković, Jasmina
Gašić, Uroš
Milutinović, Milica
Dmitrović, Slavica
Anđelković, Boban
Dimitrijević, Milena
Dragićević, Milan
Mišić, Danijela
Остала ауторства
Milutinović, MilicaТип документа:
Конференцијски прилог (Објављена верзија)
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© 2022 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
Nepeta L. is the largest genus of the Lamiaceae family, and the exclusive representative of the
subfamily Nepetoidae which produces iridoids. However, the genus Nepeta comprises both taxa
producing iridoids (iridoid aglycones-IAs and/or iridoid glucosides-IGs), and those lacking iridoids.
Combining omics-guided approach and functional genomics in elucidating the genetic background
of the iridoid diversity within the genus Nepeta, we provide novel insights into molecular
mechanisms driving the iridoid biosynthesis, and thus into chemical evolution of iridoids within
the genus Nepeta. Multidisciplinary experimental approach is here explained on the example
of iridoid synthases (IS), functionally characterized by in vitro enzymatic assays using recombinant
proteins heterologously expressed in Escherichia coli. Functional ISs are expressed in both
iridoid-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in iridoid-lacking N. nervosa
L. (NnIS). N. nervosa possesses an iridoid biosynthetic platform, but inactive one, most likely due
to the “switching off” or “silencing” of some gene(s) upstream in the pathway, which suggests that
this species has lost the ability to produce iridoids during the evolution. Furthermore, comparative
iridoid profiling and co-expression analysis of biosynthetic genes and transcription factors (TFs)
during the exposure of chemodiverse Nepeta taxa to environmental stresses (e.g. dehydration,
UV−B radiation, pathogens) or elicitors (MeJA), facilitated the explanation of regulatory mechanisms
determining the productivity of plants, as well as of factors responsible for the presence/
absence of iridoids or specific groups of iridoids (IAs and IGs), in these plants.
Кључне речи:
Nepeta; dehydration; functional characterization of genes; iridoid synthase; nepetalactoneФинансирање / пројекти:
- NEPETOME - Omics-guided disentangling of the iridoid diversity in the genus Nepeta L. for in planta metabolic engineering towards sustainable exploitation (RS-ScienceFundRS-Ideje-7749433)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- Milutinović M, editor. Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; 2022. p. 136.