dc.contributor | Milutinović, Milica | |
dc.creator | Aničić, Neda | |
dc.creator | Matekalo, Dragana | |
dc.creator | Skorić, Marijana | |
dc.creator | Božunović, Jelena | |
dc.creator | Nestorović Živković, Jasmina | |
dc.creator | Gašić, Uroš | |
dc.creator | Milutinović, Milica | |
dc.creator | Dmitrović, Slavica | |
dc.creator | Anđelković, Boban | |
dc.creator | Dimitrijević, Milena | |
dc.creator | Dragićević, Milan | |
dc.creator | Mišić, Danijela | |
dc.date.accessioned | 2024-03-01T09:51:58Z | |
dc.date.available | 2024-03-01T09:51:58Z | |
dc.date.issued | 2022 | |
dc.identifier.isbn | 978-86-912591-6-7 | |
dc.identifier.uri | http://radar.ibiss.bg.ac.rs/handle/123456789/6563 | |
dc.description.abstract | Nepeta L. is the largest genus of the Lamiaceae family, and the exclusive representative of the
subfamily Nepetoidae which produces iridoids. However, the genus Nepeta comprises both taxa
producing iridoids (iridoid aglycones-IAs and/or iridoid glucosides-IGs), and those lacking iridoids.
Combining omics-guided approach and functional genomics in elucidating the genetic background
of the iridoid diversity within the genus Nepeta, we provide novel insights into molecular
mechanisms driving the iridoid biosynthesis, and thus into chemical evolution of iridoids within
the genus Nepeta. Multidisciplinary experimental approach is here explained on the example
of iridoid synthases (IS), functionally characterized by in vitro enzymatic assays using recombinant
proteins heterologously expressed in Escherichia coli. Functional ISs are expressed in both
iridoid-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in iridoid-lacking N. nervosa
L. (NnIS). N. nervosa possesses an iridoid biosynthetic platform, but inactive one, most likely due
to the “switching off” or “silencing” of some gene(s) upstream in the pathway, which suggests that
this species has lost the ability to produce iridoids during the evolution. Furthermore, comparative
iridoid profiling and co-expression analysis of biosynthetic genes and transcription factors (TFs)
during the exposure of chemodiverse Nepeta taxa to environmental stresses (e.g. dehydration,
UV−B radiation, pathogens) or elicitors (MeJA), facilitated the explanation of regulatory mechanisms
determining the productivity of plants, as well as of factors responsible for the presence/
absence of iridoids or specific groups of iridoids (IAs and IGs), in these plants. | sr |
dc.language.iso | en | sr |
dc.publisher | Belgrade: Serbian Plant Physiology Society | sr |
dc.relation | info:eu-repo/grantAgreement/ScienceFundRS/Ideje/7749433/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS// | sr |
dc.rights | openAccess | sr |
dc.source | Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia | sr |
dc.subject | Nepeta | sr |
dc.subject | dehydration | sr |
dc.subject | functional characterization of genes | sr |
dc.subject | iridoid synthase | sr |
dc.subject | nepetalactone | sr |
dc.title | Progress in disentangling the diversity of iridoids within the genus Nepeta: surprising biosynthetic and evolutionary insights | sr |
dc.type | conferenceObject | sr |
dc.rights.license | ARR | sr |
dc.rights.holder | © 2022 by the Serbian Plant Physiology Society | sr |
dc.description.other | Milutinović M, editor. Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; 2022. p. 136. | sr |
dc.citation.spage | 136 | |
dc.type.version | publishedVersion | sr |
dc.identifier.cobiss | 74996233 | |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/17204/bitstream_17204.pdf | |
dc.citation.rank | M34 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_6563 | |