Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes
2022
Аутори:
Marković Filipović, JelenaKojić, Danijela
Miler, Marko
Karan, Jelena
Ivelja, Ivana
Matavulj, Milica
Остала ауторства
Caput Mihalić, KatarinaMičetić Stanković, Vlatka
Urlić, Inga
Mešić, Armin
Kružić, Petar
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2022 by the Hrvatsko biološko društvo
Метаподаци
Приказ свих података о документуАпстракт:
Acrylamide (AA) is carcinogen, mutagen and neurotoxic substance present in fried, roasted and
baked starch-based goods. AA is formed in Maillard reaction from asparagine and carbonyl
sources, such as reducing sugars, during thermal food processing at temperatures between
120°C and 180°C. The aim of our study was to determine whether acrylamide treatment affects
the expression and activity of glutathione-S-transferase (GST) in hepatocyte. Viability of rat
hepatoma cell line - H4IIE upon AA treatment was assessed by the MTT viability assay. Cells were
cultivated in a 96-well plate and exposed to increasing concentrations of AA (2.5–5.5 mM) for 24
h. AA concentration of 4 mM induced death of 17.62% H4IIE cells, while 4.5 mM AA caused death
of 48.45% cells. Therefore, AA concentrations of 4 mM and 4.5 mM were taken as IC20 and IC50,
respectively. H4IIE cells were treated with IC20 and IC50 of AA for 24 h. Relative mRNA
expression for GSTA2 and GSTP1 was quantified using real-time RT-PCR. Total GST activity was
determined spectrophotometrically at 340 nm using DTNB as the substrate. After AA treatments,
mRNA level for GSTA2 and GSTP1 and total GST activity increased in a concentration-dependent
manner in H4IIE cells. Only treatment with higher AA concentration (4.5 mM) proved to be
statistically significant for all three examined parameters. Our results indicate that by altering
expression and activity of GST enzyme acrylamide exerts toxic effects on hepatocytes.
Кључне речи:
acrylamide; GST activity; GST expression; MTT viability assay; H4IIE cell lineФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200125 (Универзитет у Новом Саду, Природно-математички факултет) (RS-MESTD-inst-2020-200125)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200113 (Универзитет у Нишу, Медицински факултет) (RS-MESTD-inst-2020-200113)
У:
- Caput Mihalić K, Mičetić Stanković V, Urlić I, Mešić A, Kružić P, editors. Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska. Zagreb: Hrvatsko biološko društvo; 2022. p. 231-2.