Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia
2023
Аутори:
Kolarević, StoimirAdrienn, Micsinai
Szanto-Egesz, Reka
Lukacs, Alena
Kračun-Kolarević, Margareta
Jovanović Marić, Jovana
Đorđević, Ana
Vojnović-Milutinović, Danijela
Kirschner, Alexander
Farnleitner, Andreas
Linke, Rita
Đukić, Aleksandar
Kostić, Jovana
Sunjog, Karolina
Paunović, Momir
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2023 by the Austrian Committee - International Association for Danube Research
Метаподаци
Приказ свих података о документуАпстракт:
Pollution of natural water bodies is one of the biggest challenges in developing countries, such as
Serbia, where wastewater is discharged directly into the recipients without proper treatment and
poses serious threats to surface water quality, general safety and environmental health. Numerous
pathogenic microorganisms, bacteria, viruses, protozoa and helminthes may reach surface waters
and eventually contaminate groundwater. Despites its extreme importance in this case,
implementation of wastewater-based epidemiology is a challenge for developing countries, as the
majority of households are not connected to sewerage systems. Within our research, we have placed
major focus on recipients of wastewaters as an alternative of targeting raw wastewater. During the
fourth COVID-19 wave in Serbia that started in late February 2021 multiple water samples were
collected at 12 sites at Sava and Danube Rivers in the Belgrade city area which are under the impact
of untreated wastewaters.
SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2)
and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial
faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological
parameters were analysed in parallel. Out of 44 samples analyzed, 31 were positive for at least one
of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in
surface waters in context with the large amount of epidemiological and environmental metadata can
be used as epidemiological early-warning tool in countries with poor wastewater treatment.
У:
- Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. Austrian Committee - International Association for Danube Research; 2023. p. 11.