Brain inflammation in experimental autoimmune encephalomyelitis induced in Dark Agouti rats with spinal cord homogenate
2024
Authors:
Stegnjaić, GoranJevtić, Bojan
Lazarević, Milica
Ignjatović, Đurđica
Tomić, Mirko
Nikolovski, Neda
Bjelobaba, Ivana
Momčilović, Miljana
Dimitrijević, Mirjana
Miljković, Đorđe
Stanisavljević, Suzana
Document Type:
Article (Published version)
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© 2024 Elsevier B.V.
Metadata
Show full item recordAbstract:
We have recently characterized experimental autoimmune encephalomyelitis (EAE) induced in DA rats with spinal cord homogenate without complete Freund’s adjuvant (CFA). The main advantage of this multiple sclerosis model is the lack of CFA-related confounding effects which represent the major obstacles in translating findings from EAE to multiple sclerosis. Here, antigen specificity of the cellular and humoral immune response directed against the central nervous system was explored. The reactivity of T and B cells to myelin basic protein, myelin oligodendrocyte glycoprotein, and β-synuclein was detected. Having in mind that reactivity against β-synuclein was previously associated with autoimmunity against the brain, the infiltration of immune cells into different brain compartments, i.e. pons, cerebellum, hippocampus, and cortex was determined. T cell infiltration was observed in all structures examined. This finding stimulated investigation of the effects of immunization on DA rat behavior using the elevated plus maze and the open field test. Rats recovered from EAE displayed increased anxiety-like behavior. These data support CFA-free EAE in DA rats as a useful model for multiple sclerosis research.
Keywords:
experimental autoimmune encephalomyelitis; multiple sclerosis; brain; T cells; antibodies; behaviorSource:
Immunology Letters, 2024, 267, 106852-Funding / projects:
- European Federation of Immunological Societies (EFIS, 2021 Eastern Star Award)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
Related info:
DOI: 10.1016/j.imlet.2024.106852
ISSN: 0165-2478