Poređenje antimikrobne aktivnosti ekstrakta biljke Arthemisia absinthium (pelin) sa aktivnošću komercijalnih likera
Comparison of antimicrobial potential of Arthemisia absinthium (wormwood) extract with the activity of commercial liqueurs with wormwood flavor
2018
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Authors:
Ivanov, MarijaStojković, Dejan
Kostić, Marina
Miletić, Milica
Ćirić, Ana
Petrović, Jovana
Glamočlija, Jasmina
Soković, Marina
Contributors
Soković, MarinaTešić, Živoslav
Document Type:
Conference object (Published version)
,
© 2018 by the University of Belgrade
Metadata
Show full item recordAbstract:
Arthemisia absinthium L., wormwood, is perennial bushy plant known for various health benefits. Pelinkovci is
Serbian name for the liqueurs with 28% alcohol that contain wormwood (pelin) as aromatic constituent. The study
examined two commercial liqueurs: Pelinkovac (Slatka kuća, Kragujevac, Serbia – PE) and Gorki list (Neodoljivo
bilje, Subotica, Srbija - GL). In order to determine if addition of plant extract could increase beneficial effects of these
strong drinks, microdilution method was used to examine their antimicrobial activity which has been compared with
the activity of wormwood extract dissolved in 28% ethanol (PE). Microorganisms, pathogens isolated from oral cavity,
were tested (fungi Candida albicans, Candida glabrata and Candida krusei as well as bacteria Streptococcus pyogenes
and Enterococcus faecalis). Highest antifungal potential was observed for PE with minimal inhibitory concentration
(MIC) 0.17 mg/mL. Liqueurs had similar antifungal activity with MIC in range 4.19–17.32 mg/mL. C. glabrata
clinical isolate was the most sensitive followed by strains of C. albicans (3 clinical isolates and one reference strain
C. albicans ATCC 10231), while C. krusei was the most resistant to antimicrobial treatment. Highest antibacterial
activity was observed for PE (MIC 0.34-1.36 mg/mL), with E. faecalis strain being more sensitive than S. pyogenes.
Best activity in preventing C. albicans biofilm formation was observed for SK (48.2% inhibition), GL (43.6%), PE
(26.4%). Formation of E. faecalis biofilm was inhibited for 86.0% with SK, 74.6% GL, 13.6% PE. Biofilm formed
by S. pyogenes was more resistant and GL could not prevent it from establishing, while SK prevented it for 10.3%.
PE had highest preventing ability since it inhibited S. pyogenes biofilm formation for 74.5%. Both liqueurs are able
to prevent growth of tested pathogenic microorganisms in vitro, but SK had better potential in prevention of grouping
into biofilm, indicating that we could further work on development of this drink, possibly through addition of more
wormwood since it has been proven as strong antimicrobial agent.
Funding / projects:
- Characterization and application of fungal metabolites and assessment of new biofungicides potential (RS-MESTD-Basic Research (BR or ON)-173032)
In:
- Soković M, Tešić Ž, editors. Unifood conference: Programme & Book of Abstracts; 2018 Oct 5-6; Belgrade, Serbia. Belgrade: University of Belgrade; 2018. BKHP68 / FQSP68.